研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスに見る磁界ばく露の精巣毒性影響とコエンザイムQ10およびLカルニチンの予防的役割] med./bio.

Testicular Toxicity Effects of Magnetic Field Exposure and Prophylactic Role of Coenzyme Q10 and L-Carnitine in Mice.

掲載誌: Pharmacol Res 2002; 46 (4): 363-370

この研究は、高磁界(20mT)によるマウス精巣毒性に対するL-カルニチンまたはコエンザイムQ10(CoQ10)の保護効果を評価した。動物には、磁界ばく露の1時間前に、L-カルニチン(200mg/ kg(腹腔内投与)またはCoQ10(200mg/ kg(経口投与)処置を行い、その後に磁界を分割ばく露(1日30分、週3回、2週間)または急性ばく露(3時間)した。総精子数、運動性、1日あたりの精子産生、精巣LDH-X活性、ならびに組織病理学的検査で評価を行なった。その結果、事前の薬剤投与なしに磁界の分割ばく露を行なった場合、精子数、運動性、1日あたりの精子産生、およびLDH-X活性の有意な低下が生じた;LDH-X活性の低下は、急性ばく露に比べ、より大きかった;さらに、分割ばく露後には、精巣組織の顕著な病理学的変化が観察された;磁界ばく露の1時間前にL-カルニチンまたはCoQ10でマウスを前処理すると、高磁界(20mT)がマウスに誘導する精巣損傷の著しい回復が起きた、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The detrimental effects of exposure of mice to a 50 Hz magnetic field on the testes and sperms and the protective effects of coenzyme Q10 and carnitine should be investigated.

詳細情報

A total of 90 mice were divided into 9 groups (n=10 each but only n=6 were used for analysis, respectively).
The groups 1-3 were injected with 1) saline (0.05 ml/20 g body weight, intraperitoneally), 2) coenzyme Q10 (200 mg/kg body weight, orally) or 3) carnitine (200 mg/kg body weight, intraperitoneally), respectively, 3 times per week for 2 weeks, and they were not exposed to the magnetic field (control groups).
The groups 4-6 were injected with the same doses of 4) saline, 5) coenzyme Q10 or 6) carnitine, respectively, 1 hour before repeated exposure to the magnetic field for 30 min per day three times per week for 2 weeks.
The groups 7-9 were injected with the same doses of 7) saline, 8) coenzyme Q10 or 9) carnitine, respectively, 1 hour before acute exposure to the magnetic field for 3 h.
One day after the end of exposure, animals were sacrificed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 30 min/day, 3 times a week for 2 weeks
repeated exposure
ばく露2: 50 Hz
ばく露時間: continuous for 3 h
acute exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 30 min/day, 3 times a week for 2 weeks
Additional information repeated exposure
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cage with Perspex windows blocking its two end sides allowing 12 mice to move around freely
ばく露装置の詳細 system consisted of three rectangular coils connected in parallel; current used was 3.17 A; a doll wooden frame similar to the cage dimensions was used for construction of the coils (approximate cross-section 22 cm × 12 cm) and wound with 760 turns of 1mm diameter copper wire; a cooling copper serpentine was constructed and placed in close proximate to the interior surface of the coil around the cage to control the temperature
パラメータ
測定量 種別 Method Mass 備考
磁束密度 20 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 3 h
Additional information acute exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 20 mT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Repeated (group 4) or acute (group 7) exposure of mice caused a significant decrease in sperm count, motility, daily sperm production and germ cell maturation compared to the control group (group 1). Moreover, histopathological changes (dilatation of seminiferous tubules and absence of spermatogonia and spermatogenesis) were observed in the testes after repeated exposure to the magnetic field (remark EMF-Portal: no results were presented for acute exposure to the magnetic field).
For both exposure conditions, treatment of mice with coenzyme Q10 (groups 5 and 8) or carnitine (groups 6 and 9) led to significant higher values of sperm count, motility, daily sperm production and germ cell maturation compared to the non-treated exposure groups (groups 4 and 7). Most histopathological changes induced by the repeated magnetic field exposure could be prevented by a treatment with coenzyme Q10 or carnitine.
No changes were observed between the unexposed control groups (groups 1-3).
The authors conclude that exposure of mice to a 50 Hz magnetic field might damage the testes and sperms and that coenzyme Q10 and carnitine could have a protective effect.

研究の種別:

研究助成

関連論文