研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトグリオーマ細胞のHsp70、Hsp27発現に対する1950 MHz ラジオ周波電磁界ばく露の影響] med./bio.

Effects of exposure to a 1950 MHz radio frequency field on expression of Hsp70 and Hsp27 in human glioma cells.

掲載誌: Bioelectromagnetics 2005; 26 (4): 251-257

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

To study whether exposure to a radiofrequency field could act as an environmental insult to invoke a stress response in human glioma cells, using HSP27 and HSP70 (different heat shock proteins) as stress markers.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,950 MHz
Modulation type: CW
ばく露時間: continuous for 1 or 2 h
ばく露2: 1,950 MHz
Modulation type: CW
ばく露時間: continuous for 10 or 30 min and 1 or 2 h

ばく露1

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1 or 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure apparatus consisting of two single-mode resonator cavities for 1.8 GHz (based on the R18 waveguide) was placed in an ordinary incubator.
ばく露装置の詳細 Cells cultured in 35-mm dishes were placed on dish holders in the two waveguides, one for RF and one for sham exposure. A PC randomly determined which of the two waveguides was exposed.
Sham exposure A sham exposure was conducted.
Additional information None of the exposure conditions produced a rise in temperature greater than 0.1 °C. Other cells were treated at 43 °C for 2 or 3 h as a positive control.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg - - - -
SAR 2 W/kg - - - -

ばく露2

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 10 or 30 min and 1 or 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 10 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Sham-exposed and exposed cells demonstrated a similar growth pattern up to 4 days after exposure. Exposure at both 2 and 10 W/kg did not affect the growth of the cells. In addition, there were no significant differences in protein expression of HSP27 and HSP70 between sham-exposed and exposed cells at a SAR of 1, 2, or 10 W/kg for 1 and 2 h. However, exposure at a SAR of 10 W/kg for 1 and 2 h decreased the protein level of phosphorylated Hsp27 significantly.
The data suggest that although exposure to a 1950 MHz radiofrequency field has no effect on cell proliferation and expression of HSP27 and HSP70, it may inhibit the phosphorylation of HSP27 at serine 78 in MO54 cells (HSP27 is phosphorylated at three phosphorylation sites (serine 15, serine 78, and serine 82) by protein kinases. In this investigation, an anti-HSP27 antibody that is specific for phosphorylated HSP27 (serine 78) was used).

研究の種別:

研究助成

関連論文