研究のタイプ: 医学/生物学の研究 (experimental study)

[複合無線周波放射にばく露したMCF10A細胞における細胞ストレス応答の分析] med./bio.

Analysis of the cellular stress response in MCF10A cells exposed to combined radio frequency radiation.

掲載誌: J Radiat Res 2012; 53 (2): 176-183

この細胞研究は、無線周波電磁界に対するヒト乳腺上皮細胞MCF10Aの細胞ストレス応答を種々のばく露条件について調べた。ばく露条件は、CDMAまたはCDMA+広帯域CDMAの4時間ばく露、または同じものの2時間ばく露をそれぞれ3日連続した。SARはCDMA単独では4.0 W/kg、CDMA+広帯域CDMAでは各2.0 W/kg で合計4.0 W/kgとした。特定の熱ショックタンパク(HSP)の発現レベルとリン酸化状態、分裂促進因子活性化タンパク質キナーゼMAPK)を分析した結果、熱ショックまたは電離放射線に対するストレス応答の最も敏感なマーカであるHSP27およびERK1/2のリン酸化における変化は、CDMA単独でもCDMA+広帯域CDMAでも見られなかった。HSP27およびERK1/2のリン酸化有意な変化が無いことは、MCF10A細胞のHSP27およびERK1/2の活性化が引き起こされなかったことを示すと結論している。

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研究目的(著者による)

To analyse the cellular stress response in human breast epithelial cells exposed to combined radiofrequency irradiation.

詳細情報

First, an experimentwas conducted to find the most sensitive markers of cellular stress. Therefore, cells were stressed either by heat shock (39 °C, 41 °C or 43 °C) or ionizing radiation (0,2 oder 1 Gy). Analysis by Western Blot showed that the phosphorylated forms of the proteins Hsp27 and ERK1/2 were the most sensitive markers. Hence, these proteins were taken for further analysis.
For the main experiment four experimental groups were analysed:
1.) control group in incubator
2.) sham exposed
3.) exposure at 837 MHz (4 W/kg)
4.) exposure simultaneously at 837 MHz and 1950 MHz (each 2 W/kg, in total 4 W/kg). <brThe cells were exposed either four hours one day or two hours on three consecutive days. As a positive control MCF7 human breast carcinoma cells were incubated for 1 h at 43 °C and analysed afterwards.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 837 MHz
ばく露時間: continuous for 4 h once or 2 h/day on 3 days
ばく露2: 837–1,950 MHz
ばく露時間: continuous for 4 h once or 2 h/day on 3 days
  • SAR: 4 W/kg (total)
  • SAR: 2 W/kg (each for CDMA and W-CDMA)

ばく露1

主たる特性
周波数 837 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 4 h once or 2 h/day on 3 days
Modulation
Modulation type cf. additional information
Additional information

CDMA

ばく露装置
ばく露の発生源/構造
  • Radial Transmission Line + conical antenna
ばく露装置の詳細 100 mm Petri dish containing the cells placed besides the conical antenna inside the Radial Transmission Line
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - - -

ばく露2

主たる特性
周波数 837–1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 4 h once or 2 h/day on 3 days
Modulation
Modulation type cf. additional information
Additional information

CDMA and W-CDMA

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - - total
SAR 2 W/kg - - - each for CDMA and W-CDMA

Reference articles

  • Lee KY et al. (2011): [細胞周期とその調節タンパク質に対する無線周波放射への組合せばく露の影響]
  • Pickard WF et al. (2000): [誘電体装荷放射状送電線における培養瓶内のSAR分布の実験的および数的測定]
  • Moros EG et al. (1999): [大量の培養瓶へのマイクロ波照射用広帯域遮蔽ばく露装置としての放射状送電線]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Single or combined radiofrequency field exposure did not induce phosphorylation of ERK1/2 or Hsp27 in MCF10A cells. Additionally, the levels of ERK1/2 and Hsp27 remained unaltered. The same results were obtained inconsiderably whether the cells were exposed one time for four hours or two hours on three consecutive days.
These results suggest that exposure to neither single (836.5 MHz) nor combined (836.5 MHz and 1950 MHz) radiofrequency field irradiation stimulates a stress response in MCF10A cells under the examined conditions.

研究の種別:

研究助成

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