この研究は、健常者および電磁界（EMF）過敏症の自己申告者から採取したヒトリンパ球に異なる搬送周波数のGSMシステムのマイクロ波（MW）を照射し、その影響を調べた。照射後に、ストレス応答および遺伝毒性影響の指標となるクロマチン立体構造の変化を、異常粘度時間依存法で測定した。また、DNA二重鎖切断（DSB）の指標として、腫瘍抑制因子p53結合タンパク質1（53BP1）とリン酸化ヒストンγ-H2AXが共局在するフォーカスを免疫蛍光共焦点レーザー顕微鏡で分析した。その結果、GSM携帯電話のMWは、熱ショックと同様にクロマチンの立体構造および53BP1 /γ-H2AX フォーカスに影響することが示された；この研究で初めて、ヒトリンパ球に対する携帯電話のMWの影響は、キャリア周波数に依存するが示された；しかしこのような影響に、が健常者およびEMF過敏症自己申告者のリンパ球での違いはなかった、と報告している。
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To study the effects of microwaves of Global System for Mobile Communication (GSM) at different carrier frequencies on human lymphocytes from healthy persons and from persons reporting hypersensitivity to electromagnetic fields.
Heat treatment in water bath (at 41°C and 43°C) was used as a positive control for stress response.
Several proteins, such as the tumor suppressor p53-binding protein 1 (53BP1) and the phosphorylated histone H2AX (gamma-H2AX), have been shown to produce discrete intranuclear foci, which are believed to colocolize with DNA double-strand breaks providing a scaffold structure for DNA double-strand breaks repair.
|ばく露時間||continuous for 1 h|
|Pulse width||577 µs|
Neither voice modulation nor DTX mode was applied.
|チャンバの詳細||Cells were exposed in 14 ml round-bottom tubes (2.5 ml cell suspension per tube) which were placed in a humidified CO2 incubator maintained at 37°C.|
|ばく露装置の詳細||The output of the mobile phone (see reference article) was connected via coaxial cable to a TEM cell. The power was monitored online using a power meter.|
|Additional information||In each experiment, the cells from the same blood samples were exposed in the same TEM cell in sequence and in random order to microwaves at 905 MHz and 915 MHz and sham-exposed with microwaves off. Heat treatment in a water bath, at 41°C and 43°C, was used as a positive control for stress responses. As a positive control for genotoxic effect, the cells were irradiated with 137Cs γ-rays, at 3 Gy, with a dose rate of 10.6 Gy/min.|
Microwaves from GSM mobile phones affect chromatin conformation and 53BP1/gamma-H2AX foci similar to heat shock. These effects indicate stress responses and/or DNA damage. The effects of microwaves from mobile phones on human lymphocytes are dependent on carrier frequency. On average, the same response was found in lymphocytes from hypersensitive and healthy subjects.