研究のタイプ: 医学/生物学の研究 (experimental study)

[GSM-900携帯電話信号に対するヒト皮膚細胞のストレス応答:単離初代細胞および再構成された表皮でのイン・ビトロ研究] med./bio.

Human skin cell stress response to GSM-900 mobile phone signals. In vitro study on isolated primary cells and reconstructed epidermis.

掲載誌: FEBS J 2006; 273 (24): 5491-5507

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研究目的(著者による)

To explore the effects of 900 MHz GSM radiofrequency exposure on human skin in vitro.

詳細情報

Cell cultures of isolated human skin cells and human reconstructed epidermis using human keratinocytes were exposed.
Cells exposed to UV-B radiation represented the positive controls.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 48 h
  • SAR: 2 W/kg mean
  • SAR: 16 W/kg peak

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 48 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Additional information

GSM protocol

ばく露装置
ばく露の発生源/構造
  • wire-patch antenna
チャンバの詳細 The antenna was surrounded by a foam-rubber ring and placed in a cell-culture incubator maintained at 37 ± 0.1 °C.
ばく露装置の詳細 The antenna contained eight 35-mm Petri dishes filled with 3.2 ml of medium, each placed at the centre of a 60-mm diameter Petri dish filled with 5 ml of water.
Sham exposure A sham exposure was conducted.
Additional information A twin incubator with an inactive antenna was used for sham exposure. UVB radiation was used as a positive control at 600 mJ/cm² and 200 mJ/cm² single doses with Petri dishes or 24-well plates.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg mean 計算値 - -
SAR 16 W/kg peak 計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The radiofrequency exposure did not induce apoptosis in the isolated cells and there was no alteration in the human reconstructed epidermis thickness or cell proliferation.
There was no change observed in the heat shock protein expression in the isolated keratinocytes. By contrast, a slight but significant increase in the Hsp70 expression was observed in the human reconstructed epidermis after 3 and 5 weeks of culture. Moreover, the fibroblasts showed a significant decrease in the Hsc70 expression, depending on the cell culture conditions.
These data indicate that adaptive cell behaviour in response to radiofrequency exposure, depending on the cell type and cell culture conditions, is unlikely to have deleterious effects at the skin level.

研究の種別:

研究助成

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