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Cell cultures of isolated human skin cells and human reconstructed epidermis using human keratinocytes were exposed.
Cells exposed to UV-B radiation represented the positive controls.
|ばく露時間||continuous for 48 h|
|Duty cycle||12.5 %|
|Repetition frequency||217 Hz|
|チャンバの詳細||The antenna was surrounded by a foam-rubber ring and placed in a cell-culture incubator maintained at 37 ± 0.1 °C.|
|ばく露装置の詳細||The antenna contained eight 35-mm Petri dishes filled with 3.2 ml of medium, each placed at the centre of a 60-mm diameter Petri dish filled with 5 ml of water.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||A twin incubator with an inactive antenna was used for sham exposure. UVB radiation was used as a positive control at 600 mJ/cm² and 200 mJ/cm² single doses with Petri dishes or 24-well plates.|
The radiofrequency exposure did not induce apoptosis in the isolated cells and there was no alteration in the human reconstructed epidermis thickness or cell proliferation.
There was no change observed in the heat shock protein expression in the isolated keratinocytes. By contrast, a slight but significant increase in the Hsp70 expression was observed in the human reconstructed epidermis after 3 and 5 weeks of culture. Moreover, the fibroblasts showed a significant decrease in the Hsc70 expression, depending on the cell culture conditions.
These data indicate that adaptive cell behaviour in response to radiofrequency exposure, depending on the cell type and cell culture conditions, is unlikely to have deleterious effects at the skin level.