研究のタイプ: 医学/生物学の研究 (experimental study)

[無線周波への出生前ばく露:動物モデルの胸腺細胞発達および末梢T細胞コンパートメントに対するWiFi信号の影響] med./bio.

Prenatal exposure to radiofrequencies: Effects of WiFi signals on thymocyte development and peripheral T cell compartment in an animal model.

掲載誌: Bioelectromagnetics 2012; 33 (8): 652-661

この実験研究は、妊娠初期から出産予定日の1日前までWiFi信号の電磁界(中心周波数帯2450MHz)に子宮ばく露したマウス胎仔の出生後のT細胞の発達と機能への影響を調べた。妊娠準備のできた雌マウスをそれぞれ16匹ずつ、電磁界ばく露群、擬似ばく露群、ケージ対照群として交配させたのち、ばく露群には1日2時間、SAR4 W/kgの電磁界ばく露を与えた(2個のTEMセルに8匹ずつ入れた。8匹のばく露は同等であることをモデル計算で確認した)。各群の新生仔は全て同じ環境で生育させ、同じ母マウスから生まれた仔マウス雌雄各1匹を5週目ないし26週目に屠殺して、免疫学的分析を行った。その結果、全ての群において胸腺細胞細胞数・表現型増殖に何も変化はなく、脾臓細胞数・CD4/CD8比・T細胞増殖サイトカイン産生にもばく露の影響は見られず、また雌と雄、5週目と26週目でも同じ結果であったと報告している。

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研究目的(著者による)

To study the effects of prenatal (in utero) exposure in mice to WiFi signals on development and function of immune cells.

詳細情報

The development of the immune system begins during embryogenesis, continues through fetal life, and is followed by a postnatal maturation period lasting several weeks in mice. All these phases are highly susceptible to the action of several noxious agents.
Pregnant mice were divided into three groups (n=16 per group): 1.) cage control, 2.) sham exposed and 3.) exposed. Exposure started five days after mating and ended one day before the expected delivery. The offspring was examined at the age of 5 weeks and 26 weeks. At every time point, one male and one female pup from each dam were sacrificed to perform immunological analyses.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2,451–2,473 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h/day on 14 consecutive days (starting 5 days after mating, ending 1 day before expected delivery)
  • SAR: 4 W/kg average over mass (whole body)

ばく露1

主たる特性
周波数 2,451–2,473 MHz
タイプ
  • electromagnetic field
波形
  • pulsed
特性
  • guided field
ばく露時間 continuous for 2 h/day on 14 consecutive days (starting 5 days after mating, ending 1 day before expected delivery)
Additional information single channel WiFi transmission; central frequency 2462 MHz
Modulation
Modulation type pulsed
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
チャンバの詳細 mice restrained in transparent cylindrical polymethyl-methacrylate jigs with the posterior part on the side of the field origin and maintained with the caudal axis parallel to the direction of field propagation
ばく露装置の詳細 two identical 120 cm long TEM cells; signal generated by commercial WiFi access point connected to a notebook via LAN and communicating with a PC using single channel transmission with channel 11; signal directly taken from the access point; daily clockwise rotation of the eight pregnant mice inside each of the two TEM cells used; temperature regulated; mice positioned at the two areas of maximum E-field (4 mice/area), two above and two under each of the two septums
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg average over mass 測定値および計算値 whole body -

Reference articles

  • Pinto R et al. (2010): [新生仔マウス用の2.45GHz WiFi周波数ばく露装置のドシメトリ]
  • Ardoino L et al. (2005): [電磁界の生物学的影響の研究を目的としたIn vivoパイロット試験用の無線周波システム]
  • Gatta L et al. (2003): [マウス末梢リンパ球に対するGSM変調900MHz放射線へのイン・ビボばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

No effects due to exposure to WiFi signals during pregnancy on mating success, number of newborns per dam and body weight of offspring at birth were found.
The results showed no exposure-related effects on the number and proliferation as well as on the cell differentiation in the thymus and the spleen at both time points. Observed differences were due to age and/or gender.
In summary, these results do not support the hypothesis that in utero-exposure to a WiFi signal has detrimental effects on the development of immune cells in mice.

研究の種別:

研究助成

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