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To study the effects of exposure to 900 MHz and 1800 MHz on bone fracture and the role of CAPE (caffeic acid phenethyl ester) on the induced changes.
CAPE is one of the major components of honeybee propolis. It has been used in folk medicine for many years in Middle East countries. CAPE was found to be a potent free radical scavenger and antioxidant. 48 rats were divided into five groups: 1) sham exposure (n=10); 2) 900 MHz exposure (n=8); 3) treatment with CAPE before 900 MHZ exposure (n=10) ; 4) 1800 MHz exposure (n=10); 5) treatment with CAPE before 1800 MHz exposure (n=10). At the end of the experiment, the animals were sacrificed and the left femur was removed.
bone fracture: femoral length and outer mid-shaft width; strength of femoral shaft (three-point bending test using a mechanical testing device: values of total fracture energy (load and maximal deflection), breaking force, bending strength)
The exposure groups that were pretreated with CAPE had significantly higher values in breaking force, bending strength, and total fracture engery of the femur compared to the exposure groups without CAPE pretreatment. However, all exposure and CAPE treatment groups (2-5) showed decreased values for all three parameters compared to the sham exposed group (1). Both radiofrequency electromagnetic fields and CAPE had effects on bones.