研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界はラットの脳内の一酸化窒素シグナリングを変調させる] med./bio.

Extremely low-frequency magnetic fields modulate nitric oxide signaling in rat brain.

掲載誌: Bioelectromagnetics 2012; 33 (7): 568-574

この実験研究は、超低周波磁界(ELF-MF)がラットの脳におけるCa2+依存性NO合成酵素NOS)によるNO合成誘導することを示した前回の研究結果を確認するために、脳の各部位における神経型NOS(nNOS)への影響およびNOとNOS活性の相関を調べた。ばく露群、擬似ばく露群(各10匹)は、ヘルムホルツコイルによる2mT、60Hz の磁界擬似ばく露群は電源オフ)に5日間ばく露した。その結果、ばく露群では、cGMP上昇を伴うNOレベル上昇が大脳皮質、線状体、海馬で見られたが、それらの部位におけるニューロンの形態異常および数に有意な変化はなかった。一方、これらの部位におけるnNOS-免疫反応性ニューロンの数はばく露群で有意に上昇したことを報告している。

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研究目的(著者による)

The study was designed to confirm that an extremely low frequency magnetic field affects neuronal nitric oxide synthase (nNOS) in several brain regions and to investigate the correlation between nitric oxide and nNOS activation.

詳細情報

A previous study showed that an extremely low frequency magnetic field induces nitric oxide synthesis by Ca2+-dependent NO synthase in the rat brain (Jeong et al. 2006). In the central nervous system, nitric oxide derived from nNOS acts as a neuromodulator or neurotransmitter for the regulation of synaptic plasticity, the sleep-wake cycle and hormone secretion.
10 rats were exposed and 10 rats were sham exposed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 5 days
  • 磁束密度: 2 mT effective value (at the center of the cage)
  • 磁束密度: 1.8 mT effective value (at the corner of the cage)

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 5 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 pair of Helmholtz coils, consisting of 200 turns of insulated copper wire with a diameter of 1 mm, wound on a 140 cm x 85 cm x 70 cm rectangular wooden frame; each winding was split; maximum magnetic field in the center of the coil system; non-magnetic cage placed in the center
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT effective value 測定値 - at the center of the cage
磁束密度 1.8 mT effective value 測定値 - at the corner of the cage

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The exposure of the rats to the magnetic field for 5 days resulted in significant increases of the nitric oxide level in the cerebral cortex, striatum, and hippocampus and in a significant elevation of the cGMP level in the striatum in comparison to the control group. There were no significant differences in the morphology and number of neurons in the cerebral cortex, striatum, and hippocampus. However, the number of nNOS-immunoreactive neurons were significantly increased in those cerebral areas in exposed rats.
These data suggest that the increase in nitric oxide could be due to the increased expression and activation of nNOS. In conclusion, the extremely low frequency magnetic field exposure was able to increase the nitric oxide production via nNOS activation in the brain of the rats.

研究の種別:

研究助成

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