研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスでの抑うつ障害に対する短期および長期の超低周波磁界の影響:一酸化窒素経路の関与] med./bio.

Effects of short term and long term extremely low frequency magnetic field on depressive disorder in mice: involvement of nitric oxide pathway.

掲載誌: Life Sci 2016; 146: 52-57

この研究は、NMRI成獣雄マウスに超低周波磁界(ELF MF:0.5 mT、50Hz)の単回の2時間ばく露(短期ばく露)、または2時間/日の2週間ばく露(長期ばく露)を与え、ばく露終了後にオープンフィールド試験での自発運動量(活動性の指標)、強制水泳試験での無動時間(抑うつ様状態の指標)を測定した。その結果、短期ばく露では無動時間に変化はなかった;長期ばく露では、無動時間が有意に短縮した;一方、自発運動量には両方のばく露による違いが見られなかった、と報告した。

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研究目的(著者による)

The effects of exposure of mice to a 50 Hz magnetic field on depressive-like behavior and the role of nitric oxide should be investigated.

詳細情報

NG-nitroarginine methyl ester (L-NAME) was used to exert an anti-depressive effect. It was diluted in physiological saline and administered intraperitoneally (30 mg/kg) after exposure.
72 male mice were divided into the following groups (n=9 each): 1) acute exposure, 2) chronic exposure, 3) acute exposure and administration of L-NAME, 4) acute exposure and administration of saline (sham administration). For each group, a separate sham exposure group was used.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 2 hours
acute exposure
ばく露2: 50 Hz
ばく露時間: continuous for 2 hours/day for 2 weeks (6 days/week, 1 day break)
chronic exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 hours
Additional information acute exposure
ばく露装置
ばく露の発生源/構造
チャンバの詳細 plexiglass cage
ばく露装置の詳細 two pairs of coaxially arranged Helmholtz coils with winding embedded in a plexiglass frame; the diameter of each coil was 91.4 cm; the distance between the coils in the pairs was 50 cm and there were 60 turns of 2.5 mm copper wire oriented along the vector of the geomagnetic field; the centrally horizontal MF was homogenous in the plexiglass cage with 4-5 animals (groups were divided); the temperature during exposure did not exceed 27 °C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT effective value 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 hours/day for 2 weeks (6 days/week, 1 day break)
Additional information chronic exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT effective value 測定値 - -

Reference articles

  • de Bruyn L et al. (2010): [マウスの受精率に対する無作為に変動する50Hz電力周波数磁界への長期ばく露の影響]
  • Lee BC et al. (2004): [マウス脳の抗酸化防御系への低周波磁界の影響:化学発光研究]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Chronic exposure (group 2) significantly reduced depressive-like behaviour compared to the sham exposure, while acute exposure (group 1) did not show significant differences compared to the sham exposure.
However, acute exposure in combination with L-NAME (group 3) significantly increased the depressive-like behaviour compared to the respective sham exposure and thus negating the anti-depressing effect of L-NAME.
Locomotor activity did no show any significant differences between the groups.
The authors conclude that chronic exposure of mice to a 50 Hz magnetic field could alter depressive-like behavior in mice. The reversion of the anti-depressive effect of L-NAME indicates a possible increase of nitric oxide in the brain due to the magnetic field exposure.

研究の種別:

研究助成

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