研究のタイプ: 医学／生物学の研究 (experimental study)

[超低周波磁界（50Hz、0.5mT）は全脳虚血後のスナネズミの脳における酸化ストレスを低減する] med./bio.

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

著者: Raus Balind S, Selakovic V, Radenovic L, Prolic Z, Janac B
掲載誌: PLoS One 2014; 9 (2): e88921
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この研究は、実験的に2本の総頸動脈を閉鎖し、一時的に（10分間）全脳虚血にさせた成獣（3月齢）のスナネズミに、超低周波磁界（ELF-MF：50Hz、0.5mT）のばく露を与え、脳のストレスを調べた。(1) 無処置群（n=6）：手術、ELF-MFばく露の両方無し、(2) ELF-MF群（n=13）および(3) 擬似ばく露群（n=6）：ELF-MFに7日間連続ばく露（擬似ばく露）、(4) 脳虚血群（n=12）：結紮手術で10分間の脳虚血後に再灌流、(5) 脳虚血＋ELF-MF群（n=13）：10分間の脳虚血後、再灌流して、ELF-MFに7日間連続ばく露、(6) 擬似手術群（n=6）：同様の手術手技は行ったが脳虚血は起こしていない、の生化学的分析（脳の各組織の一酸化窒素とスーパーオキシドアニオンの産生、スーパーオキシドジスムターゼ活性、脂質過酸化指標）を再灌流の7日後、14日後に行った。その結果、脳虚血自体は、7日後、14日後において、脳の酸化ストレスを増加させた；ELF-MF群では、酸化ストレスが増加し、その程度は、7日後においてのみ、脳虚血より大きかった；脳虚血＋ELF-MF群では、7日後に酸化ストレス増加したが、その程度は脳虚血単独またはELF-MF単独より小さかった；再灌流から14日後では、酸化ストレスはほとんどが無処置群レベルに回復した、と報告している。

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研究目的（著者による）

To investigate whether extremely low frequency magnetic fields influence oxidative stress in the brain of gerbils submitted to global cerebral ischemia.

詳細情報

Gerbils were submitted to 10 minutes of global cerebral ischemia by occlusion of both carotid arteries. Afterwards, animals were exposed to an extremely low frequency magnetic field for 7 days and examined either directly after the exposure (7. experimental day, immediate effect of exposure) or 7 days later (14. experimental day, delayed effects of exposure).
The following groups were examined: 1.) cage control (n=6), 2.) sham operated without ischemia (n=6), 3.) sham exposed (n=6), 4.) exposure (n=13), 5.) ischemia (n=12) and 6.) ischemia + exposure (n=13).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 7 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7 days
ばく露装置
ばく露の発生源／構造
  • electromagnet
ばく露装置の詳細 electromagnet was placed in an isolated room with same conditions as vivarium (temperature 23 ± 2°C; humidity 55 ± 10%; lights on for 12 hours daily (7-19 Uhr))
Sham exposure A sham exposure was conducted.
Additional information geomagnetic activity was characterized as "very quiet" (measured by the Department of Geomagnetism and Aeronomy, Serbia)
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.5 mT - - - -

Reference articles

  • Raus S et al. (2012): [超低周波磁界は全脳虚血を呈するスナネズミの運動行動に変化を生じる]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No differences were found between the cage control, sham operated and sham exposed group. Hence, these gerbils were considered as the only control group (n= 8).
In all examined brain areas of gerbils submitted to ischemia, oxidative stress parameters were significantly increased on the 7. and 14. experimental day compared to the control group. In magnetic field-exposed gerbils, oxidative stress parameters were significantly increased compared to the control group on the 7. day, but not on the 14. day. Ischemic gerbils that were exposed had significantly increased oxidative stress levels on the 7. day compared to the control, but to a lesser extent than gerbils submitted to ischemia or magnetic field-exposure alone. However, on the 14. experimental day, oxidative stress parameters from gerbils of this group were similar to the parameters of the control group.
Summarized, magnetic field-exposure or submission to cerebral ischemia alone led to an increase in the oxidative stress levels in the brain of gerbils, while a combination of both treatments diminished the effect. The authors conclude that the results could indicate a beneficial effect of extremely low frequency magnetic fields in the management of global cerebral ischemia.

研究の種別:

研究助成

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