研究のタイプ: 医学/生物学の研究 (experimental study)

[変調および非変調の2450 MHz無線周波電磁界にばく露したヒト末梢血リンパ球での小核の出現頻度] med./bio.

Incidence of micronuclei in human peripheral blood lymphocytes exposed to modulated and unmodulated 2450 MHz radiofrequency fields.

掲載誌: Bioelectromagnetics 2013; 34 (7): 542-548

この研究は、4人の喫煙歴のない健康なドナー(男3人、女1人)から採取した末梢血標本での小核の出現頻度に対する変調および非変調の2450MHz無線周波の非変調(CW)および変調(WCDMA)の電磁界RF)のインビトロばく露(2時間)の影響を調べた。ばく露は、培養フラスコを導波管内に並べて行い、モデル計算によるフラスコ内培養液の平均SARは10.9 W/kgであった。1.5 Gyガンマ線急性ばく露をポジティブコントロールとした。各ドナーの標本を、C(培養器コントロール)、S(擬似ばく露)、RF、M(メラトニン添加)、RF+M、S+Mの6群で2通り(RFがCWおよびWCDMAの場合)の計12群に分け、実験1日目はCW、2日目はWCDMAの実験を行った。2時間のばく露終了から全てのフラスコのリンパ球を72時間培養して小核MN)出現頻度を評価した。その結果、C、S、RF、M群のMN出現頻度は同等であった;WCDMAとCW 群に有意差はなかった;ガンマ線によるポジティブコントロールでは有意なMN出現頻度の上昇を示した;メラトニン処置はRFばく露群、擬似ばく露群に効果を示さなかったが、ガンマ線群ではMN出現頻度を減少させる効果を示した、と報告している。

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研究目的(著者による)

To study the extent of genetic damage in human peripheral blood lymphocytes exposed to modulated wideband code division multiple access (WCDMA) and continuous wave (CW) radiofrequency exposures and whether the damage, if any, can be prevented by treatment of the cells with melatonin.

詳細情報

Blood of four healthy volunteers was collected. Gamma radiation (1.5 Gy) was used as positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2,450 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h
  • 電力: 2.57 W
  • SAR: 10.9 W/kg (across the four flasks (average values in each flask: 11.7, 12.7, 10.4 and 8.8 W/kg))
ばく露2: 2,450 MHz
Modulation type: CW
ばく露時間: continuous for 2 h
  • 電力: 2.57 W
  • SAR: 10.9 W/kg (across the four flasks (average values in each flask: 11.7, 12.7, 10.4 and 8.8 W/kg))

General information

Aliquots of blood samples were treated under the following conditions: 1. untreated control, left in an incubator 2. melatonin alone (2 mM), left in an incubator 3. radiofrequency alone 4. melatonin + radiofrequency exposure 5. sham exposure 6. melatonin + sham exposure

ばく露1

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Additional information

W-CDMA modulated

ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 open-ended waveguide (108 mm wide, 54 mm high, 605 mm long) operating in the TE10 mode and producing vertically polarized plane wave conditions; entire exposure apparatus was kept in a warm room maintained at a temperature of 35°C; four flasks (36 mm x 66 mm) containing blood samples were arranged horizontally, with the longest dimension perpendicular to the longest dimension of the waveguide, and the height of the medium in the flasks aligned vertically with the electric field; flask closest to the antenna was placed at a distance of around 270 mm from the antenna; each subsequent flask was placed 10 mm from the neighboring flask
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 2.57 W - - - -
SAR 10.9 W/kg - - - across the four flasks (average values in each flask: 11.7, 12.7, 10.4 and 8.8 W/kg)

ばく露2

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 2.57 W - - - -
SAR 10.9 W/kg - - - across the four flasks (average values in each flask: 11.7, 12.7, 10.4 and 8.8 W/kg)

Reference articles

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The results indicated that the incidence of micronuclei was similar in incubator controls and in samples exposed to radiofrequency/sham exposure and melatonin alone. There were no significant differences between W-CDMA and CW radiofrequency exposures. Melatonin treatment had no effect on cells exposed to radiofrequency and sham exposure, while such treatment significantly reduced the frequency of micronuclei in gamma radiation-exposed cells.

研究の種別:

研究助成

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