研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界はATM-Chk2-p21経路の活性化を通してG1期停止を引き起こす] med./bio.

Extremely Low-Frequency Electromagnetic Fields Cause G1 Phase Arrest through the Activation of the ATM-Chk2-p21 Pathway.

掲載誌: PLoS One 2014; 9 (8): e104732

この研究は、不死化したヒト表皮ケラチノサイト細胞株HaCaTに対する超低周波電磁界(ELF-EMFs:60 Hz、1.5 mT)の144時間ばく露の影響を調べた。その結果、ELF-EMFsばく露により、G1期停止は生じ、コロニー形成が低下した;ATM-Chk2信号伝達カスケードの活性化が誘導された、などを報告している。

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研究目的(著者による)

To investigate the biological effects of exposure to extremely low frequency magnetic fields in HaCaT cells at the transcriptional, protein and cellular level.

詳細情報

Ionizing radiation (UV-B, 233 J/m²) was performed as a positive control.
Further controls were performed using siRNA (small interfering RNA), which is a class of double-stranded RNA molecules, 20-25 base pairs in length and do not code for proteins. It is most notable in the interference of the expression of specific genes with complementary nucleotide sequences resulting in a prevention of translation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)
  • 磁束密度: 1.5 mT (± 0.03 µT, in the central area of the coils)

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 pair of coil apparatuses each had a diameter of 34 cm and were 17 cm apart. AC power to the Helmholtz coil system was supplied by a step-down transformer connected to a 60-Hz, 110 V AC source
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - 測定値 - ± 0.03 µT, in the central area of the coils

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In 144 hours-exposed cell cultures, the number of cells in the G1 phase was significant increased ("G1 phase cell cycle arrest") compared to the sham exposure, while the cell proliferation and colony forming units were significantly decreased. The protein expression of phosphorylated ATM, phosphorylated Chk2 and p21 was higher in the exposed cell cultures (after 96, 120 and 144 hours) than in the sham exposed ones. The magnetic field-induced G1 phase cell cycle arrest was diminished when the gene expression of CHK2 was prevented by transfection with the siRNA.
The authors conclude that extremely low frequency magnetic fields can trigger cell cycle arrest in the G1 phase in HaCaT cells. Furthermore, they conclude that the results indicate an involvement of an ATM-Chk2-p21 pathway.

研究の種別:

研究助成

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