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To investigate the biological effects of exposure to extremely low frequency magnetic fields in HaCaT cells at the transcriptional, protein and cellular level.
Ionizing radiation (UV-B, 233 J/m²) was performed as a positive control.
Further controls were performed using siRNA (small interfering RNA), which is a class of double-stranded RNA molecules, 20-25 base pairs in length and do not code for proteins. It is most notable in the interference of the expression of specific genes with complementary nucleotide sequences resulting in a prevention of translation.
ばく露時間: continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)
|ばく露時間||continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)|
|磁束密度||1.5 mT||-||測定値||-||± 0.03 µT, in the central area of the coils|
In 144 hours-exposed cell cultures, the number of cells in the G1 phase was significant increased ("G1 phase cell cycle arrest") compared to the sham exposure, while the cell proliferation and colony forming units were significantly decreased. The protein expression of phosphorylated ATM, phosphorylated Chk2 and p21 was higher in the exposed cell cultures (after 96, 120 and 144 hours) than in the sham exposed ones. The magnetic field-induced G1 phase cell cycle arrest was diminished when the gene expression of CHK2 was prevented by transfection with the siRNA.
The authors conclude that extremely low frequency magnetic fields can trigger cell cycle arrest in the G1 phase in HaCaT cells. Furthermore, they conclude that the results indicate an involvement of an ATM-Chk2-p21 pathway.