研究のタイプ: 医学/生物学の研究 (experimental study)

[1800 MHz無線周波数電磁界ばく露は初代培養ニューロンのミトコンドリアDNAに酸化的損傷を生じる] med./bio.

Exposure to 1800 MHz radiofrequency radiation induces oxidative damage to mitochondrial DNA in primary cultured neurons.

掲載誌: Brain Res 2010; 1311: 189-196

ミトコンドリアDNA(mtDNA)欠損とさまざまな神経系疾患との間には密接な関係があり、またmtDNAは酸化ストレスに極めて弱いことに着目して、この実験研究ではRF電磁界によるmtDNAの酸化損傷(一般的バイオマーカーは8-ヒドロキシグアニン(8-OHdG)を指標として)を評価した。用いた細胞初代培養皮質ニューロンばく露は217 Hzでパルス変調した1800 MHz電磁界SAR=2 W/kg、ばく露時間24時間とした。その結果、8-OHdGの有意な濃度上昇、mtDNAのコピー数とミトコンドリアRNA転写産物のレベルの顕著な減少が見られたと報告している。

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研究目的(著者による)

To study whether radiofrequency irradiation can cause oxidative damage to mitochondrial DNA (mtDNA) in primary cultured cortical neurons of rats.

詳細情報

mtDNA defects are closely associated with various nervous system diseases and mtDNA is particularly susceptible to oxidative stress.
The cells were assigned to one of five groups: (1) sham exposure group, (2) melatonin group, (3) radiofrequency exposure group, (4) radiofrequency exposure+melatonin group, (5) hydrogen peroxide exposure (100 µM for 24 h, as a positive control of oxidative stress). In groups (2) and (4), melatonin was freshly diluted with cell medium before application (4 h prior to radiofrequency exposure, at a concentration of 200 nM). Groups (2) and (5) were put into the incubator for sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: 5 min on - 10 min off - for 24 hr
  • SAR: 2 W/kg average over mass

General information

cells were treated in five groups: i) sham exposure ii) Melatonin iii) RF exposure iv) RF exposure + Melatonin (4 hr prior to exposure) v) H2O2 exposure for 24 hr

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 5 min on - 10 min off - for 24 hr
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg average over mass - - -

Reference articles

  • Zhao R et al. (2007): [1800 MHz無線周波数電磁界ばく露のラット神経細胞における遺伝子発現のcDNAマイクロアッセイによる研究]
  • Diem E et al. (2005): [イン・ビトロでのヒト線維芽細胞および形質転換GFSH-R17ラット顆粒膜細胞における携帯電話放射(1800MHz)による非熱的なDNA切断]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to radiofrequency irradiation for a period of 24 h significantly increased the production of reactive oxygen species compared with sham exposure group. Thus, radiofrequency exposure induced oxidative stress in the neurons, and this effect could be reversed by melatonin pretreatment.
At 24 h after exposure, radiofrequency exposure induced a significant increase in the levels of 8-hydroxy-2-deoxyguanosine in the mitochondria of neurons. Concomitant with this finding, the copy number of mitochondrial DNA and the levels of mitochondrial RNA transcripts showed an obvious reduction after radiofrequency exposure. These findings further proved that radiofrequency exposure damaged mitochondrial DNA in neurons. Each of these mitochondrial DNA disturbances could be reversed by pretreatment with the antioxidant melatonin.
The authors conclude that these results suggested that 1800 MHz radiofrequency exposure could cause oxidative damage to mitochondrial DNA in primary cultured neurons.

研究の種別:

研究助成

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