研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁ノイズはラジオ周波数照射で誘導されたヒト水晶体上皮細胞にDNAダメージと活性酸素種増加を抑制する] med./bio.

Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells.

掲載誌: Mol Vis 2008; 14: 964-969

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研究目的(著者による)

To study the influence of the 1.8 GHz radiofrequency fields (GSM) on DNA damage and intracellular reactive oxygen species formation in human eye lens epithelial cells and whether the effects induced by radiofrequency could be blocked by superposing of electromagnetic noise (2 µT).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: intermittent, 5 min on/10 min off, for 2 h
  • SAR: 1 W/kg mean
  • SAR: 2 W/kg mean
  • SAR: 3 W/kg mean
  • SAR: 4 W/kg mean
ばく露2: 30–90 Hz
ばく露時間: intermittent, 5 min on/10 min off, for 2 h

General information

The cells were subjected to one of four exposure conditions: sham, RF radiation (4 levels), noise MF, and RF radiation superposed with noise MF.

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 5 min on/10 min off, for 2 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM signal

ばく露装置
ばく露の発生源/構造
チャンバの詳細 Two waveguides, one for RF and one for sham exposure, were placed inside a conventional incubator at 37 °C with 5% CO2 and 95% air.
ばく露装置の詳細 Six 35-mm Petri dishes with cells in a total volume of 2 ml were placed in a dish holder inside the waveguide holding them exactly in the H-field maximum of the standing wave and were exposed simultaneously in E polarization.
Sham exposure A sham exposure was conducted.
Additional information The system enabled the exposure of a monolayer of cells with less than 30% non-uniformity of the SAR.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg mean - - -
SAR 2 W/kg mean - - -
SAR 3 W/kg mean - - -
SAR 4 W/kg mean - - -

ばく露2

主たる特性
周波数 30–90 Hz
タイプ
  • magnetic field
ばく露時間 intermittent, 5 min on/10 min off, for 2 h
Additional information white noise signal
ばく露装置
ばく露の発生源/構造
チャンバの詳細 To generate a noise MF, both sides of the waveguides were wrapped with two rectangular Helmholtz coils having a center distance of 24 cm.
Additional information The direction of the coils was the same as the circular wires in the RF waveguides, and the direction of the noise MF was consistent with the magnetic field of the RF radiation.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 µT - - - "Amplitude"

Reference articles

  • Yao K et al. (2008): [水晶体上皮細胞でのマイクロ波誘発性DNA損傷に対するマイクロ波と電磁界ノイズの重ね合わせた効果]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After exposure to 1.8 GHz radiofrequency electromagnetic field for 2 h, the cells exhibited significant intracellular reactive oxygen species production increase in the 2, 3, and 4 W/kg groups. Radiofrequency exposure at the SAR of 3 W/kg and 4 W/kg could induce significant DNA damage in the alkaline comet assay, while no statistical difference in double strand breaks was found between exposure (3 and 4 W/kg) and sham exposure groups.
When radiofrequency was superposed with 2 µT electromagnetic noise could block radiofrequency-induced reactive oxygen species increase and DNA damage.
In conclusion, DNA damage induced by 1.8 GHz radiofrequency field may be associated with the increased reactive oxygen species production. Electromagnetic noise could block this radiofrequency-induced intracellular reactive oxygen species formation and DNA damage.

研究の種別:

研究助成

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