The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
To investigate the effects of exposure to extremely low frequency magnetic fields on the oxidative stress response in a human neuroblastoma cell line.
The human neuroblastoma cell line SH-SY5Y is used as an cellular model to examine the potential influence of extremely low frequency magnetic fields on cellular cascades and signal pathways that may lead to neurodegeneration. This cell line is believed to possess a number of physiological systems that have parallels to human neurons.
To examine whether extremely low frequency magnetic fields may influence the oxidative homeostasis of neural cells, the cell cultures were partially treated with H2O2 (co-exposure).
|ばく露時間||up to 24 hours (1 h, 3 h, 6 h or 24 h)|
|ばく露装置の詳細||a 160 turn solenoid (22 cm length, 6 cm radius, copper wire diameter of 1.25 x 10-5 cm) that generated a horizontal magnetic field; solenoid was placed inside the exposure incubator (5% CO2 atmosphere and 37°C ± 0.3°C)|
|磁束密度||1 mT||effective value||測定値||-||-|
No significant differences regarding cell morphology and cell viability were observed between exposed and control cell cultures. However, in cell cultures exposed for 1, 3, 6, or 24 hours, the enzyme activity of the nitric oxide synthase and the production of superoxide were significantly increased compared to the control group, while the enzyme activity of the catalase was only significantly increased after 6 hours of exposure. Furthermore, an exposure to magnetic fields changed enzymatic kinetic parameters of the catalase and the cytochrome P450: The exposure induced a modest increase in the total velocity of the catalase, accompanied by a moderate rise in the minimum velocity and an elevated "rate of decrease" compared to the control. Regarding the cytochrome P450, after exposure the "rate of increase" was significantly elevated and the "rate of decrease" was increased in comparison to the control.
The gene expression levels of the transforming growth factor beta-1 and the interleukin-18-binding protein were significantly increased in exposed cell cultures when compared to control cell cultures.
In H2O2-treated cultures, the enzyme activity of the catalase was significantly decreased after 24 hours magnetic field exposure compared to incubation without magnetic field exposure.
The authors conclude that exposure to extremely low frequency magnetic fields does not have an impact on the cell viability but could induce oxidative stress in human neuroblastoma cells.