研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界ばく露への神経細胞の応答:酸化ストレスと神経変性に関する意味合い] med./bio.

Neuronal cellular responses to extremely low frequency electromagnetic field exposure: implications regarding oxidative stress and neurodegeneration.

掲載誌: PLoS One 2014; 9 (8): e104973

この研究は、不死神経細胞モデルであるヒトSH-SY5Y細胞株に対する磁界(50Hz、1mT)の影響を調べた。その結果、磁界ばく露群でNOSおよびO2-の発現が上昇した;また、サイトカイン遺伝子発現の変化も見られた、と報告している。

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研究目的(著者による)

To investigate the effects of exposure to extremely low frequency magnetic fields on the oxidative stress response in a human neuroblastoma cell line.

詳細情報

The human neuroblastoma cell line SH-SY5Y is used as an cellular model to examine the potential influence of extremely low frequency magnetic fields on cellular cascades and signal pathways that may lead to neurodegeneration. This cell line is believed to possess a number of physiological systems that have parallels to human neurons.
To examine whether extremely low frequency magnetic fields may influence the oxidative homeostasis of neural cells, the cell cultures were partially treated with H2O2 (co-exposure).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: up to 24 hours (1 h, 3 h, 6 h or 24 h)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 up to 24 hours (1 h, 3 h, 6 h or 24 h)
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 a 160 turn solenoid (22 cm length, 6 cm radius, copper wire diameter of 1.25 x 10-5 cm) that generated a horizontal magnetic field; solenoid was placed inside the exposure incubator (5% CO2 atmosphere and 37°C ± 0.3°C)
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT effective value 測定値 - -

Reference articles

  • Vianale G et al. (2008): [超低周波電磁界はヒトケラチノサイト細胞の増殖促進および炎症促進性ケモカイン産生減少を引き起こす]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences regarding cell morphology and cell viability were observed between exposed and control cell cultures. However, in cell cultures exposed for 1, 3, 6, or 24 hours, the enzyme activity of the nitric oxide synthase and the production of superoxide were significantly increased compared to the control group, while the enzyme activity of the catalase was only significantly increased after 6 hours of exposure. Furthermore, an exposure to magnetic fields changed enzymatic kinetic parameters of the catalase and the cytochrome P450: The exposure induced a modest increase in the total velocity of the catalase, accompanied by a moderate rise in the minimum velocity and an elevated "rate of decrease" compared to the control. Regarding the cytochrome P450, after exposure the "rate of increase" was significantly elevated and the "rate of decrease" was increased in comparison to the control.
The gene expression levels of the transforming growth factor beta-1 and the interleukin-18-binding protein were significantly increased in exposed cell cultures when compared to control cell cultures.
In H2O2-treated cultures, the enzyme activity of the catalase was significantly decreased after 24 hours magnetic field exposure compared to incubation without magnetic field exposure.
The authors conclude that exposure to extremely low frequency magnetic fields does not have an impact on the cell viability but could induce oxidative stress in human neuroblastoma cells.

研究の種別:

研究助成

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