研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト上皮幹細胞の増殖に対する低周波電磁界の影響:インビトロ研究] med./bio.

Effects of low frequency electromagnetic field on proliferation of human epidermal stem cells: An in vitro study.

掲載誌: Bioelectromagnetics 2013; 34 (1): 74-80

この研究は、ヒト上皮幹細胞(hESC)の増殖に対する低周波磁界の影響をin vitroで実験した。磁界は、1、10、50 Hz の5 mT、ばく露時間は30 min/日で、それぞれ3、5、7 日間とした。その結果、磁界ばく露はhESCの増殖を周波数依存的に有意に高め、50Hzで最も増殖速度が高かった;磁界ばく露はS期細胞の割合増加(G1期細胞の割合減少とともに)を有意に示したが、周波数依存的ではなかった;CD29+/CD71-の割合に変化はなかった、などの所見を報告している。

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研究目的(著者による)

To examine the effects of low frequency electromagnetic fields on the proliferation of epidermal stem cells.

詳細情報

Three different frequencies (1 Hz, 10 Hz and 50 Hz) and a sham exposed control were tested.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1 Hz
ばく露時間: continuous for 30 min/day on 3, 5, or 7 days
  • 磁束密度: 5 mT effective value (at the center of the coils)
ばく露2: 10 Hz
ばく露時間: continuous for 30 min/day on 3, 5, or 7 days
  • 磁束密度: 5 mT effective value (at the center of the coils)
ばく露3: 50 Hz
ばく露時間: continuous for 30 min/day on 3, 5, or 7 days
  • 磁束密度: 5 mT effective value (at the center of the coils)

ばく露1

主たる特性
周波数 1 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 min/day on 3, 5, or 7 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 pair of Helmholtz coils with a mean radius of 15 cm ± 0.3 cm positioned vertical to the horizontal plane at a horizontal distance of 15 cm ± 0.5 cm; cells placed in plates with six wells in the coil system; field uniformity approximately 90%; exposure system placed in an incubator with a constant temperature of 37°C ± 0.5°C, 5% CO2 and 50% humidity
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT effective value 測定値 - at the center of the coils

ばく露2

主たる特性
周波数 10 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 min/day on 3, 5, or 7 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT effective value 測定値 - at the center of the coils

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 min/day on 3, 5, or 7 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT effective value 測定値 - at the center of the coils

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No influence of magnetic field-exposure on the cell morphology and on the cell phenotype were detected in any of the groups. In all exposed cell cultures, the cell proliferation was significantly increased compared to the sham exposed cultures, in a frequency dependent manner, with the highest rate in the 50 Hz-group. Magnetic field-exposure led to a significant increase of cells in the S phase and to a significant decrease of cells in the G1 phase. The changes in the cell cycle distibution were not dependent on the frequency.
The data indicate a promoting influence of low frequency magnetic fields on the proliferation of human epidermal stem cells.

研究の種別:

研究助成

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