この研究は、ヒト上皮幹細胞（hESC）の増殖に対する低周波磁界の影響をin vitroで実験した。磁界は、1、10、50 Hz の5 mT、ばく露時間は30 min/日で、それぞれ3、5、7 日間とした。その結果、磁界ばく露はhESCの増殖を周波数依存的に有意に高め、50Hzで最も増殖速度が高かった；磁界ばく露はS期細胞の割合増加（G1期細胞の割合減少とともに）を有意に示したが、周波数依存的ではなかった；CD29+/CD71－の割合に変化はなかった、などの所見を報告している。
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To examine the effects of low frequency electromagnetic fields on the proliferation of epidermal stem cells.
|ばく露1: 1 Hz||
|ばく露2: 10 Hz||
|ばく露3: 50 Hz||
|ばく露時間||continuous for 30 min/day on 3, 5, or 7 days|
|ばく露装置の詳細||pair of Helmholtz coils with a mean radius of 15 cm ± 0.3 cm positioned vertical to the horizontal plane at a horizontal distance of 15 cm ± 0.5 cm; cells placed in plates with six wells in the coil system; field uniformity approximately 90%; exposure system placed in an incubator with a constant temperature of 37°C ± 0.5°C, 5% CO2 and 50% humidity|
|Sham exposure||A sham exposure was conducted.|
No influence of magnetic field-exposure on the cell morphology and on the cell phenotype were detected in any of the groups. In all exposed cell cultures, the cell proliferation was significantly increased compared to the sham exposed cultures, in a frequency dependent manner, with the highest rate in the 50 Hz-group. Magnetic field-exposure led to a significant increase of cells in the S phase and to a significant decrease of cells in the G1 phase. The changes in the cell cycle distibution were not dependent on the frequency.
The data indicate a promoting influence of low frequency magnetic fields on the proliferation of human epidermal stem cells.