研究のタイプ: 医学／生物学の研究 (observational study)

[超低周波磁界（ELF-MF）に職業ばく露した溶接工における一次DNA損傷] med./bio.

Primary DNA damage in welders occupationally exposed to extremely-low-frequency magnetic fields (ELF-MF)

著者: Villarini M, Dominici L, Fatigoni C, Levorato S, Vannini S, Monarca S, Moretti M
掲載誌: Ann Ig 2015; 27 (3): 511-519
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電気アーク溶接には相当な超低周波（ELF）磁界ばく露が生じることから、この研究は、アーク溶接中のELF磁界ばく露とこれに関連する遺伝毒性ハザードを評価した。ばく露群の溶接工21人および非ばく露対照群（健康な血液提供者）を対象とした。勤務時間（AM7-PM5）を通じて装着した個人ドシメータで職業ばく露を測定した。標準的なアルカリコメットアッセイで末梢血リンパ球の一次DNA損傷の程度を測定した。その結果、コメットのテイル長はばく露群と対照群で同様であったが、ばく露群では対照群と比較して、テイル強度（p=0.01）およびテイルモーメント（p=0.02）のカウントは有意に低下した。著者らは、陽性結果を報告した先行研究とこの研究との違いは、クロムおよび／またはニッケル（あるいはその他の金属）へのばく露レベルの違い（より低い濃度ではDNA－タンパク質の架橋、より高い濃度ではDNA単鎖切断につながる）によるものかも知れない、と結論付けている。

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研究目的（著者による）

The genotoxic effects in the blood of welders occupationally exposed to 50 Hz magnetic fields should be investigated.

詳細情報

Two groups of male subjects were used (n=21 each): 1) an exposure group comprising electric arc welders and 2) a control group comprising non-exposed subjects matched for age, residence and smoking habit.
Blood samples from exposed subjects were collected at the end of the work-shift after at least four consecutive days of work simultaneously with the samples from the control group.
For each subject, 50 cells per slide from at least three replicate slides were investigated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: repeated daily exposure (occupational, work-shift from 7 am to 5 pm)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 repeated daily exposure (occupational, work-shift from 7 am to 5 pm)
ばく露装置
ばく露の発生源／構造
  • welding equipment
ばく露装置の詳細 exposed subjects were from two welding companies in Perugia (Italy), both of which use metal active gas (MAG) welding processes, with a 80/20 % mixture of argon and CO2; occupational exposure to ELF-MF was measured using personal dosimeters placed in a shirt pocket or on a belt; measures were taken every 20 seconds over the 10-hour period
Additional information the exposure of subjects in the non-occupationally-exposed control group was not monitored
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.03 µT minimum 測定値 - ± 0.01 µT
磁束密度 170.78 µT maximum 測定値 - ± 20.71 µT
磁束密度 7.81 µT mean 測定値 - ± 1.39 µT
Additional parameter details

the exposure was measured continuously during one complete work-shift (7 am to 5 pm)

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Tail intensity and tail moment were significantly lower in the exposure group compared to the control group. There were no differences in the tail length between both groups.
The authors conclude that there was less DNA damage in the blood of welders occupationally exposed to 50 Hz magnet fields than in probands without this occupational exposure.

研究の種別:

研究助成

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