研究のタイプ: 医学/生物学の研究 (experimental study)

[3xTg-ADマウスにおいてELF-MF長期ばく露は認知障害を改善し、高リン酸化タウタンパク質を減少させる] med./bio.

Long-term exposure to ELF-MF ameliorates cognitive deficits and attenuates tau hyperphosphorylation in 3xTg AD mice.

掲載誌: Neurotoxicology 2016; 53: 290-300

この研究では、アルツハイマー病(AD)のトランスジェニックマウス(3xTg-ADマウス:タウタンパク質突然変異とある遺伝子突然変異発現し、6月齢で認知障害を発症する)を用いて、ADのマーカである高リン酸化タウタンパク質に対する超低周波磁界ばく露(ELF-MF:50Hz、500μT:3ヵ月間)の影響を擬似ばく露と比較した。その結果、ELF-MFばく露群では、認知障害が改善し、シナプスタンパク質が増加した;さらに、ばく露群では高リン酸化タウタンパク質が減少した、と報告している。

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研究目的(著者による)

The effects of exposure of Alzheimer's disease model mice to a 50 Hz magnetic field on cognition, oxidative stress and gene expression in the brain should be investigated.

詳細情報

Transgenic mice (3xTg strain), with an overexpression of the amyloid precursor protein and Tau protein (two proteins associated with Alzheimer's disease), that develop cognitive deficits at 6 months of age were used as an Alzheimer's disease model.
The following groups were used (n=6 mice, respectively): 1) exposure of transgenic mice to the magnetic field, 2) sham exposure of transgenic mice, 3) exposure of non-transgenic mice (129/C57BL/6 strain), 4) sham exposure of non-transgenic mice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 20 hours/day for 3 months

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 20 hours/day for 3 months
ばく露装置
ばく露の発生源/構造
  • bronze wire
チャンバの詳細 room without additional electronic devices (e.g. computers) and no lamps in close proximity to the exposure system
ばく露装置の詳細 there were five layers in the exposure device (200 cm x 70 cm x 200 cm); inside the exposure device, there was a plastic rack (140 cm x 70 cm x 200 cm) with four floors; two beams of bronze wires oriented in the same direction (current direction) created a uniform magnetic field and were affixed tightly to the framework to decrease vibration and noise; when the system was on, the sound and temperature generated were similar to those of the normal environmental values
Sham exposure A sham exposure was conducted.
Additional information for sham exposure, the two wire beams of the setup were oriented in opposite directions
パラメータ
測定量 種別 Method Mass 備考
磁束密度 500 µT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The functionality of the Alzheimer's disease model was confirmed by significant cognitive deficits, significantly decreased gene expression of memory-related synaptic proteins as well as significantly increased apoptosis, oxidative stress and levels of total and phosphorylated Tau protein in sham exposed transgenic mice (group 2) compared to sham exposed non-transgenic mice (group 4).
Exposed transgenic mice (group 1) showed significantly better cognitive skills and a significantly increased gene expression of some memory-related synaptic proteins (GLUR2, NR1, PSD95) as well as significantly less apoptosis and oxidative stress compared to the transgenic sham exposure group.
Exposed transgenic mice also showed significantly less total and phosphorylated Tau protein compared to the transgenic sham exposure group. This decrease was accompanied by a significant decrease of phosphorylated GSK3β (inactive form) CDK5 activators p25 and p35 and a significant increase of PP2Ac, indicating that effects of the magnetic field exposure may be caused by inhibition of GSK3β and CDK5 and activation of PP2Ac.
Exposure to the magnetic field had no significant effect on non-transgenic mice (group 3) compared to sham exposed non-transgenic mice.
The authors conclude that exposure of Alzheimer's disease model mice to a 50 Hz magnetic field might ameliorate cognitive deficits and attenuate tau hyperphosphorylation. This might be of use in the therapy of Alzheimer's disease.

研究の種別:

研究助成

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