研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトMono Mac 6細胞での非熱的レベル無線周波電磁界および超微粒子へのばく露の後のHsp70発現およびフリーラジカル放出] med./bio.

Hsp70 expression and free radical release after exposure to non-thermal radio-frequency electromagnetic fields and ultrafine particles in human Mono Mac 6 cells.

掲載誌: Toxicol Lett 2006; 161 (1): 73-82

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研究目的(著者による)

To study if exposure to ultrafine particles (12-14 nm, 100 µg/ml) and radiofrequency electromagnetic fields (continuous wave (CW) or modulated (217 Hz or GSM-non DTX (switched-off GSM-DTX mode))), alone or in combination influences levels of the superoxide radical anion or the stress protein heat-shock protein (HSP70) in the human monocyte cell line Mono Mac 6.

詳細情報

Heat treatment (42-43°C, 1h) was used as positive control for both stress reaction and for heat development in the radiofrequency exposure setup.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 60 min
  • SAR: 2 W/kg unspecified
ばく露2: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 60 min
  • SAR: 2 W/kg unspecified
ばく露3: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 60 min
GSM non-DTX signal
  • SAR: 2 W/kg unspecified

General information

Cells were treated in parallel as follows: non-treated (incubator control); sham exposed; RF exposed; sham + UFP; RF + UFP; and exposed to heat (42-43 °C) (positive control). UFP stands for ultrafine particles (<0.1 µm) produced by combustion processes. In this study, UFP of 12-14 nm (100 µg/ml) were used.

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 60 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 The RF exposure setup described in the reference article consisted of two single-mode resonator cavities (equipped with a fan) that were placed in an incubator with a humidified atmosphere of 5% CO2 in air maintained at 37 °C.
ばく露装置の詳細 Six 35 mm Petri dishes per cavity were placed in a dish holder positioning them in the H-field maxima of the standing waves.
Additional information Exposure and sham conditions were blindly assigned to the two cavities by the computer controlled signal unit. The temperature difference between sham and field exposed cultures never exceeded 0.3 °C. The incubator controls and the heat-treated samples were placed in the same incubator but outside of the cavities.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg unspecified 指定なし - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 60 min
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg unspecified 指定なし - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 60 min
Additional information GSM non-DTX signal
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM non-DTX signal with every 26th frame idle, which added an 8 Hz modulation component to the signal. The crest factor (ratio of pulse power and average power) was 8.3.

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg unspecified 指定なし - -

Reference articles

  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that the cells are capable to internalise ultrafine particles, and that this phagocytic activity is connected to an increased release of free radicals. This increase (40-45% above negative control) was stronger than the effect of heat treatment.
None of the employed radiofrequency exposures showed any effects on free radical levels.
Co-exposure of radiofrequency and ultrafine particles did not potentiate the ultrafine particles effect either.
The results revealed a significantly increased HSP70 expression level by heat treatment in a time-dependent manner, whereas ultrafine particles, radiofrequency, or ultrafine particles + radiofrequency were without any effect. Therefore, the authors conclude that in the investigated Mono Mac 6 cells, radiofrequency irradiation alone or in combination with ultrafine particles cannot influence stress-related responses.

研究の種別:

研究助成

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