研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトSH-SY5Y神経芽腫細胞に化学的に誘導された活性酸素種産生およびDNA損傷の872MHz無線周波電磁放射による増強] med./bio.

Enhancement of chemically induced reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells by 872 MHz radiofrequency radiation.

掲載誌: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2009; 662 (1-2): 54-58

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研究目的(著者による)

To study the effects of 872 MHz radiofrequency irradiation at a relatively high SAR value (5 W/kg; isothermal condition) on intracellular reactive oxygen species production and whether a possible effect is linked to DNA damage.

詳細情報

Co-exposure to radiofrequency irradiation and menadione, a chemical inducing intracellular reactive oxygen species production and DNA damage, was also performed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 872 MHz
Modulation type: CW
ばく露時間: continuous for 1 h
ばく露2: 872 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h

General information

cells were treated in four groups: i) menadione only ii) RF only iii) RF + menadione iv) sham RF exposure

ばく露1

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

ばく露2

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

Reference articles

  • Höytö A et al. (2006): [適度の温度上昇はL929細胞のODC活性に影響する:低レベルのラジオ周波数では影響は見られない]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

"Continuous wave radiofrequency + menadione" exposure increased DNA breakage in comparison to the cells exposed to menadione only. Comparison of the same groups also showed that reactive oxygen species level was higher in cells exposed to radiofrequency irradiation (CW) at 30 and 60 min after the end of exposure. Radiofrequency exposure alone did not induce DNA damage.
No effects of the GSM signal were found for neither reactive oxygen species production nor DNA damage.
Radiofrequency exposure (with or without menadione) did not significantly affect cell viability.
The data suggest that 872 MHz continuous wave radiofrequency exposure at 5 W/kg might enhance chemically induced reactive oxygen species production and thus cause secondary DNA damage. However, there is no known mechanism that would explain such effects from continuous wave radiofrequency exposure but not from GSM modulated radiofrequency irradiation at identical SAR.

研究の種別:

研究助成

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