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To study cell viability and reactive oxygen species formation in human lymphoblastoid cells exposed to 1950 MHz, UMTS signal, for short (1 h) or long (24 h) exposure duration. To investigate if radiofrequency exposure may modify reactive oxygen species production induced by a well known oxidative stress inducer, co-exposures with ferrous ions (Fe2+, administered as iron sulfate FeSO4) were also carried out (concurrent or after radiofrequency exposure).
Ferrous ions act by producing a very reactive free radical, the hydroxyl radical (OH.). Positive controls were treated with iron sulfate for the same duration.
|ばく露時間||5 min - 60 min or 24 h|
|ばく露装置の詳細||rectangular short-circuited waveguide (109.2 mm x 54.6 mm x 500 mm) in TE10 mode; cell cultures placed on a plastic stand inside the waveguide; electric field parallel to the sample surface|
|Sham exposure||A sham exposure was conducted.|
The data indicate that non-thermal radiofrequency exposures do not increase spontaneous reactive oxygen species formation in any of the experimental conditions investigated. Consistent with the lack of reactive oxygen species formation, no change in cell viability was found in radiofrequency exposed cells.
Similar results were obtained when co-exposures were considered: combined exposures to radiofrequency and iron sulfate (as stress-inducer) did not increase reactive oxygen species production induced by the chemical treatment alone.