【目的】900MHzの無線周波数(RF)放射にばく露されたマウスL929線維肉腫細胞における活性酸素種の誘導を、強い環境発がん物質である3-クロロ-4-(ジクロロメチル)-5-ヒドロキシル基-2(5H)-フラノン(MX)の共ばく露を行う場合と行わない場合について調べること。【方法】900MHzRFには連続波とGSM携帯電話信号の両方が用いられた。ばく露は比吸収率0.3W/kgと1W/kgで、10分間または30分間行われた。同時偽ばく露が各ばく露条件に対して行われた。MX処置は、毒性水準下レベルである500μMで、最初の10分間あるいは30分間のRFばく露中に行われた。活性酸素種の形成を、ばく露直後からばく露後1時間までの異なる収集期間において追跡した。【結果】900MHzRFばく露は、単独であってもMXとの組み合わせであっても、全ての実験条件において、活性酸素種形成を誘発することを示さなかった。一方、MXへのばく露は、調査された全ての処置時間について活性酸素種形成を統計的に有意に増加させたことから、MXはL929細胞における酸化ストレス誘導因子であることが確認された。
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To study the induction of reactive oxygen species in murine cells exposed to radiofrequency irradiation at 900 MHz, with or without co-exposure to the drinking water mutagen and carcinogen 3-chloro-4-dichloromethyl-5-hydroxy-2-5H-furanone (MX).
3-chloro-4-dichloromethyl-5-hydroxy-2-5H-furanone (MX) is a potent bacterial mutagen produced during chlorination of drinking water.
MX treatment was performed at a subtoxic level of 500 µM, and the radiofrequency field exposure was carried out during the first 10 or 30 min of MX treatment. The formation of reactive oxygen species was investigated at different times until one hour after radiofrequency exposure/co-exposure.
Six conditions were tested for each RF signal: sham exposure and two levels of RF radiation, each alone and together with MX treatment.
| 周波数 | 900 MHz |
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| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 10 or 30 min |
| Modulation type | CW |
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| ばく露の発生源／構造 |
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| チャンバの詳細 | The exposure chamber was made of a rectangular waveguide (124 x 248 x 500 mm) and two coax-to-waveguide adapters and was terminated on a matched load. |
| ばく露装置の詳細 | Two T-75 cell culture flasks containing 15 ml of medium were inserted into the waveguide. The narrow side of the waveguide and the E field were horizontal, i.e., parallel to the liquid layer (2 mm thick) in the rectangular plastic flask. |
| Sham exposure | A sham exposure was conducted. |
| Additional information | The temperature of the waveguide was maintained at 37.0 ± 0.1°C by thermostatted water circulating in a 30-m-long plastic tube (8 mm inner diameter) wound around the waveguide and kept in contact by wooden boards. A polyurethane foam cover was used for thermal insulation. |
MX treatment alone resulted in a statistically significant increase in the formation of reactive oxygen species for all the treatment durations investigated, confirming that MX is an inductor of oxidative stress in these cells.
But the data provided no evidence that 900 MHz radiofrequency exposure, either alone or in combination with MX, induced formation of reactive oxygen species under any of the experimental conditions investigated.
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