研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯電話マイクロ波によるSH-SY5Y(ヒト神経芽腫細胞株)における熱ショックタンパク質の応答の変調] med./bio.

Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves.

掲載誌: World J Biol Chem 2012; 3 (2): 34-40

この研究は、ニューロン細胞(レチノイン酸誘導によりヒト神経芽細胞腫SH-SY5Y細胞分化させたもの)に対する1800MHzマイクロ波ばく露(2時間または4時間)の影響を調べた。その結果、細胞の生育能力、Hsp27発現カスパーゼ-3活性に有意な変化は認められなかった;どちらのばく露時間でもHsp20発現有意な低下が認められたが、Hsp70のレベルは4時間ばく露のみで有意に上昇した、などの所見を報告している。

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研究目的(著者による)

To study the effects of radiofrequency exposure on the stress response in neuroblastoma cells, differentiated to dopaminergic neuron-like cells (induced by retinoic acid).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,760 MHz
ばく露時間: continuous for 2 h and 4 h

ばく露1

主たる特性
周波数 1,760 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h and 4 h
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
  • Samsung E1130
Distance between exposed object and exposure source 3 cm
Sham exposure A sham exposure was conducted.
Additional information no increase of temperature was measured around the cultures during exposure
パラメータ
測定量 種別 Method Mass 備考
SAR 0.086 W/kg - 計算値 - average
電界強度 25 V/m maximum 測定値 - -
磁界強度 66 mA/m maximum 測定値 - -
電力密度 1.7 W/m² maximum 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Under these experimental conditions, neither apoptosis nor Hsp27 expression nor caspase 3 expression were significantly changed following exposure. However, a significant decrease in Hsp20 expression was found at both times of exposure (2 and 4 h), whereas Hsp70 levels were significantly increased only after 4 h exposure (according to the abstract; within the results section, the authors also demonstrate a significant increase after the 2 h exposure compared with the sham exposed cells, remark EMF-Portal). Additionally, p-Hsp27 (phosphorylated Hsp27) expression was significantly increased in the exposed cells compared with the controls.
Remark EMF-Portal: The data on the cell viability are not clearly documented according to significance (in the abstract and discussion section: no change; within the results section: decrease), c.f. full text link.
The changes in the expression of the heat shock proteins in neuronal cells could be an early response to the microwave exposure.

研究の種別:

研究助成

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