研究のタイプ: 医学/生物学の研究 (experimental study)

[In vitroでの神経細胞のマイクロ波照射:アポトーシス研究]. med./bio.

Microwave exposure of neuronal cells in vitro: Study of apoptosis.

掲載誌: Int J Radiat Biol 2006; 82 (4): 267-275

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

To study whether apoptosis is induced in vitro in cells from a human neuroblastoma cell line by exposure to radiofrequency electromagnetic fields (GSM and continuous wave).

詳細情報

Heat controls were performed at 39°C. Apoptosis rate was assessed immediately or 24 h after exposure using three different methods.
Positive controls were performed with the protein kinase inhibitor staurosporine.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 24 h
  • SAR: 2 W/kg mean (unspecified)
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 h
  • SAR: 0.25 W/kg mean (unspecified)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • wire-patch cell (WPC)
チャンバの詳細 The WPC [Laval et al., 2000] was placed into a Plexiglas "cage" with RF absorbing ferrite walls. Sham-exposed dishes were placed in the same conditions in a second non-activated WPC in the same incubator at 37°C, 5% CO2. Control cells were placed in a second, identical incubator.
ばく露装置の詳細 The WPC allowed simultaneous and equal exposure of eight 35-mm Petri dishes containing 2 ml of complete medium (four columns of two dishes). To improve the SAR homogeneity, each dish was placed into another 50 mm dish containing 5 ml of distilled water.
Sham exposure A sham exposure was conducted.
Additional information Since a temperature increase of 2°C was detected during CW exposure due to the absorbing material placed around the WPC, heat control cells were exposed to 39°C, 5% CO2.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg mean 指定なし unspecified -

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 h
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 217 Hz
Additional information

GSM signal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
Additional information For GSM exposure, no increase of temperature was detected.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.25 W/kg mean 指定なし unspecified -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

These data showed that under the conditions of the present experiment microwave exposure (either continuous wave or GSM-900) does not significantly increase the apoptosis rate in the human neuroblastoma cell line SH-SY5Y, either 0 h or 24 h after exposure.

研究の種別:

研究助成

関連論文