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To study whether apoptosis is induced in vitro in cells from a human neuroblastoma cell line by exposure to radiofrequency electromagnetic fields (GSM and continuous wave).
Heat controls were performed at 39°C. Apoptosis rate was assessed immediately or 24 h after exposure using three different methods. Positive controls were performed with the protein kinase inhibitor staurosporine.
The WPC [Laval et al., 2000] was placed into a Plexiglas "cage" with RF absorbing ferrite walls. Sham-exposed dishes were placed in the same conditions in a second non-activated WPC in the same incubator at 37°C, 5% CO2. Control cells were placed in a second, identical incubator.
The WPC allowed simultaneous and equal exposure of eight 35-mm Petri dishes containing 2 ml of complete medium (four columns of two dishes). To improve the SAR homogeneity, each dish was placed into another 50 mm dish containing 5 ml of distilled water.
A sham exposure was conducted.
Since a temperature increase of 2°C was detected during CW exposure due to the absorbing material placed around the WPC, heat control cells were exposed to 39°C, 5% CO2.
These data showed that under the conditions of the present experiment microwave exposure (either continuous wave orGSM-900) does not significantly increase the apoptosis rate in the human neuroblastoma cell line SH-SY5Y, either 0 h or 24 h after exposure.