研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト精子の運動性、DNA断片化、クラステリン遺伝子発現に対する携帯電話放射のインビトロでの影響] med./bio.

In vitro effect of cell phone radiation on motility, DNA fragmentation and clusterin gene expression in human sperm.

掲載誌: Int J Fertil Steril 2015; 9 (1): 129-136

この研究は、被験者(n=124)から採取した精子運動性、DNA断片化、クラステリン(CLU)遺伝子発現に対する携帯電話からの無線周波電磁界RF-EMFばく露のイン・ビトロでの影響を調べた。まず採取した精子を分析し、正常精子(N群n=26)、精子無力症(A群n=32)、精子奇形無力症(AT群n=31)、乏精子精子奇形無力症(OAT群n=35)に分け、試各サンプルは2分されて、RF-EMFばく露および無ばく露処置を1時間受けた。ばく露には携帯電話を用いた。処置の前および直後に、精子運動性、アクロシン活性、DNA断片化、クラステリン遺伝子発現を測定した。その結果、無ばく露群に比べ、ばく露群では精子運動性、アクロシン活性が低下し、DNA断片化、クラステリン遺伝子発現が増加した;その影響の大きさは、OAT群>AT群>A群>N群であった、と報告している。

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研究目的(著者による)

The effects of exposure of human sperm to an 850 MHz electromagnetic field from a mobile phone on different sperm parameters should be investigated.

詳細情報

Clusterin is a protein, which is overexpressed in stressed tissues and appears to be potentially related pathophysiological processes. Acrosin is the major proteinase present in the acrosome of mature spermatozoa, and it is important for fertilization.
Semen samples were collected from 124 donors. Each sample was analyzed in respect of the semen quality according to WHO guidelines. Afterwards, samples were divided into the following groups: 1) normal sperm quality (n=26), 2) decreased sperm motility (n=32), 3) decreased sperm motility and increased rate of sperm malformations (n=31) and 4) severely decreased sperm motility and malformations of sperms (n=35). Each sample was divided into two portions: one for exposure to the electromagnetic field and one without exposure as the control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 850 MHz
ばく露時間: continuous for 60 minutes

General information

all samples were kept and exposed at room temperature

ばく露1

主たる特性
周波数 850 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 60 minutes
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
Distance between exposed object and exposure source 10 cm
パラメータ
測定量 種別 Method Mass 備考
電力 1 W maximum - - -
SAR 1.46 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In group 1 (normal sperm quality), the gene expression and protein expression of clusterin as well as the DNA fragmentation were significantly increased in sperms exposed to the electromagnetic field compared to the control group.
In all other groups (2-4, with pre-experimental sperm quality deficiencies), sperm motility and acrosin enzyme activity were significantly decreased and the gene expression and protein expression of clusterin as well as the DNA fragmentation were significantly increased compared to the control group. A trend towards enhanced effects in sperms with more severe pre-experimental sperm quality deficiencies could be observed.
The authors conclude that exposure of human sperm to an 850 MHz electromagnetic field from a mobile phone could have detrimental effects on sperms.

研究の種別:

研究助成

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