研究のタイプ: 医学/生物学の研究 (experimental study)

[50Hz磁界へのばく露はEP受容体介在性のPKC経路を通して小脳顆粒ニューロンでのGABA電流を変化させる] med./bio.

Exposure to 50 Hz magnetic field modulates GABA currents in cerebellar granule neurons through an EP receptor-mediated PKC pathway.

掲載誌: J Cell Mol Med 2015; 19 (10): 2413-2422

この研究は、ラット小脳顆粒ニューロン(CGNs)のγ-アミノ酪酸タイプA受容体(GABAA Rs)に対する50Hz磁界(ELF-MF:1 mT、60分間ばく露)の影響を調べた。まず、ELF-MFばく露でGABAA R電流は有意に上昇するが、GABAへの感受性に変化はないことが示されたことを踏まえ、このような影響へのEP受容体介在性のPKC経路の関与を探索するために行った種々の実験の結果を報告している。

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研究目的(著者による)

The effects of exposure of rat cerebellar granule neurons to 50 Hz magnetic fields on gamma aminobutyric acid (GABA) type A receptor ion channel activity and associated signal pathways should be investigated.

詳細情報

Cells were divided into the following groups: 1) exposure to a magnetic field of 0.2 mT for 60 and 120 minutes, 2) exposure to a magnetic field of 1 mT for 30 and 60 minutes, 3) sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for up to 120 min
ばく露2: 50 Hz
ばく露時間: continuous for up to 60 min

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for up to 120 min
ばく露装置
ばく露の発生源/構造
チャンバの詳細 culture plates
ばく露装置の詳細 a pair of horizontal Helmholtz coils (20 cm in height and 20 cm in radius, each coil consisting of 150 turns of copper wire) was placed parallel to each other; the surfaces of the culture plates were perpendicular to the force lines of the alternating magnetic field; the magnetic field was homogeneous in exposure area
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.2 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for up to 60 min
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

Reference articles

  • Wu X et al. (2014): [弱い商用周波磁界は上皮成長因子刺激に類似した作用として、ヒト羊膜細胞の上皮成長因子受容体感受性アクチン細胞骨格の運動性を急激に活性化させる]
  • Varani K et al. (2012): [ラットの脳のアデノシン受容体に対するパルス電磁界ばく露の影響]
  • Ongaro A et al. (2012): [電磁界(EMF)およびアデノシン受容体はヒト骨関節炎の滑膜線維芽細胞におけるプロスタグランジンE2およびサイトカインの放出を変化させる]
  • De Mattei M et al. (2009): [ウシ滑膜線維芽細胞内のプロスタグランジンE2放出を抑制するアデノシン類似体および電磁界]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cells exposed to a magnetic field of 0.2 mT (group 1) for 120 min or of 1 mT (group 1) for 60 min showed significantly increased GABA type A receptor ion channel currents compared to sham exposed cells. No significant differences were obeserved at the respective earlier points in time. An activation of protein kinase A led to a slight decrease of the ion channel current, however, whereas a protein kinase C activation led to an increase of the currents comparable to the magnetic field exposure and the inhibition of protein kinase C blocked the current.
Western blot analysis indicated that the protein expression of phosphorylated protein kinase C was significantly increased in cells after 60 minutes of 1 mT exposure in comparison to sham exposed cells, which could subsequently be blocked by the addition of the prostaglandin E receptor inhibitor SC19220. SC19220 also significantly inhibited the magnetic field-induced elevation of the GABA type A receptors ion channel currents.
The authors conclude that exposure of rat cerebellar granule neurons to a 50 Hz magnetic field could increase the GABA type A receptor ion channel activity and that these effects might be mediated via prostaglandin E receptor 1-protein kinase C pathway.

研究の種別:

研究助成

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