この研究は、ヒトケラチノサイト細胞株NCTC2544の活性酸素種（ROS）産生に対する超低周波電磁界（ELF-EMFs：50 Hz、磁束密度0.25 - 2 G）の1時間ばく露の影響を調べた。ばく露終了後の時間経過による変化も観察した。その結果、0.5および1 Gばく露により有意なROSレベル上昇が生じた；この2つの磁束密度のばく露後の経過観察では、抗酸化防御および細胞膜完全性のパラメータの変化に磁束密度による傾向の違いが見られ、特に0.5 Gばく露の1時間後において細胞感受性が高かった；全ての観察された変化は、ばく露後24時間で回復した､と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
The effects of exposure of human keratinocytes to a 50 Hz magnetic field on cell viability, oxidative stress and the antioxidative defense system should be investigated.
The study was conducted to examine possible cellular damage from the usage of magnetic fields in skin wound healing.
Cells were divided into 6 groups: exposure to a magnetic field of 1) 0.025 mT, 2) 0.05 mT, 3) 0.1 mT, 4) 0.15 mT, 5) 0.2 mT and 6) sham exposure. In two approaches, 3 µM phenanthroline or 2 µM rotenone were added 30 min before 1 hour of exposure in group 2. Phenanthroline is an iron chelator which prevents the Fenton reaction in the cells, while rotenone is an inhibitor of the mitochondrial respiratory chain.
Cells were either investigated directly after exposure or 24 hours later. Positive control was performed.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 1 hour
|
|
|
ばく露2:
50 Hz
ばく露時間:
continuous for 1 h, 2 h or 4 hours
|
|
|
ばく露3:
50 Hz
ばく露時間:
continuous for 1 h, 2 h or 4 hours
|
|
|
ばく露4:
50 Hz
ばく露時間:
continuous for 1 hour
|
|
|
ばく露5:
50 Hz
ばく露時間:
continuous for 1 hour
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 hour |
| ばく露の発生源／構造 |
|
|---|---|
| ばく露装置の詳細 | the system consisted of three parallel copper plates (40 x 40 cm) and 4 rectangular containers filled with circulating distilled water to regulate temperature (maintained at 37 ± 0.3°C); Faraday shields excluded electric noise in the units |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.025 mT | - | 測定値 | - | - |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 hour |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.15 mT | - | 測定値 | - | - |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 hour |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.2 mT | - | 測定値 | - | - |
Only exposure to a 0.05 mT (group 2) and 0.1 mT (group 3) magnetic field for 1 or 2 hours (but not 4 hours) resulted in significantly increased oxidative stress compared to sham exposed cells. Hence, further parameters were only investigated in these groups.
In group 2, phenanthroline reversed the oxidative stress effect while rotenone did not show any effect.
Lipid peroxidation was significantly increased in group 2 after 1 hour, while the amount of reduced glutathione and enzyme activities of glutathione reductase and superoxide dismutase were significantly decreased compared to the sham exposure group. The effects attenuated over time and after 24 hours, there were no significant differences between exposed and sham exposed cells found anymore. Group 3 showed a significantly reduced superoxide dismutase enzyme activity after 1 hour and a significantly increased amount of reduced glutathione after 4 hours of exposure compared to the sham exposure group.
Cell viability was not influenced significantly by exposure to a magnetic field in any group.
The authors conclude that exposure of human keratinocytes to a 50 Hz magnetic field might induce slight oxidative stress that does not overwhelm the antioxidative capacity of the cells and can be compensated without showing a cytotoxic effect.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
