研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトケラチノサイト細胞株NCTC2544の抗酸化活性に対する超低周波電磁界の影響] med./bio.

Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.

掲載誌: Biotechnol Appl Biochem 2017; 64 (3): 415-422

この研究は、ヒトケラチノサイト細胞株NCTC2544の活性酸素種ROS)産生に対する超低周波電磁界(ELF-EMFs:50 Hz磁束密度0.25 - 2 G)の1時間ばく露の影響を調べた。ばく露終了後の時間経過による変化も観察した。その結果、0.5および1 Gばく露により有意なROSレベル上昇が生じた;この2つの磁束密度ばく露後の経過観察では、抗酸化防御および細胞膜完全性のパラメータの変化に磁束密度による傾向の違いが見られ、特に0.5 Gばく露の1時間後において細胞感受性が高かった;全ての観察された変化は、ばく露後24時間で回復した、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The effects of exposure of human keratinocytes to a 50 Hz magnetic field on cell viability, oxidative stress and the antioxidative defense system should be investigated.

詳細情報

The study was conducted to examine possible cellular damage from the usage of magnetic fields in skin wound healing.
Cells were divided into 6 groups: exposure to a magnetic field of 1) 0.025 mT, 2) 0.05 mT, 3) 0.1 mT, 4) 0.15 mT, 5) 0.2 mT and 6) sham exposure. In two approaches, 3 µM phenanthroline or 2 µM rotenone were added 30 min before 1 hour of exposure in group 2. Phenanthroline is an iron chelator which prevents the Fenton reaction in the cells, while rotenone is an inhibitor of the mitochondrial respiratory chain.
Cells were either investigated directly after exposure or 24 hours later. Positive control was performed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 1 hour
ばく露2: 50 Hz
ばく露時間: continuous for 1 h, 2 h or 4 hours
ばく露3: 50 Hz
ばく露時間: continuous for 1 h, 2 h or 4 hours
ばく露4: 50 Hz
ばく露時間: continuous for 1 hour
ばく露5: 50 Hz
ばく露時間: continuous for 1 hour

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源/構造
  • copper plates
ばく露装置の詳細 the system consisted of three parallel copper plates (40 x 40 cm) and 4 rectangular containers filled with circulating distilled water to regulate temperature (maintained at 37 ± 0.3°C); Faraday shields excluded electric noise in the units
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.025 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 h, 2 h or 4 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.05 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 h, 2 h or 4 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.15 mT - 測定値 - -

ばく露5

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.2 mT - 測定値 - -

Reference articles

  • Potenza L et al. (2012): [白トリュフ(セイヨウショウロ子実体)に対する300mTの静磁界および50Hz、0.1mTの超低周波磁界の影響]
  • Miller DL et al. (1989): [既存の極低周波電界ばく露システムへの磁界ばく露システムの追加]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Only exposure to a 0.05 mT (group 2) and 0.1 mT (group 3) magnetic field for 1 or 2 hours (but not 4 hours) resulted in significantly increased oxidative stress compared to sham exposed cells. Hence, further parameters were only investigated in these groups.
In group 2, phenanthroline reversed the oxidative stress effect while rotenone did not show any effect.
Lipid peroxidation was significantly increased in group 2 after 1 hour, while the amount of reduced glutathione and enzyme activities of glutathione reductase and superoxide dismutase were significantly decreased compared to the sham exposure group. The effects attenuated over time and after 24 hours, there were no significant differences between exposed and sham exposed cells found anymore. Group 3 showed a significantly reduced superoxide dismutase enzyme activity after 1 hour and a significantly increased amount of reduced glutathione after 4 hours of exposure compared to the sham exposure group.
Cell viability was not influenced significantly by exposure to a magnetic field in any group.
The authors conclude that exposure of human keratinocytes to a 50 Hz magnetic field might induce slight oxidative stress that does not overwhelm the antioxidative capacity of the cells and can be compensated without showing a cytotoxic effect.

研究の種別:

研究助成

関連論文