研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界による免疫機能障害に対するハスの莢抽出成分プロシアニジンの保護効果] med./bio.

Protective effect of procyanidins extracted from the lotus seedpod on immune function injury induced by extremely low frequency electromagnetic field.

掲載誌: Biomed Pharmacother 2016; 82: 364-372

この研究は、マウス実験で超低周波電磁界(ELF-EMF:50Hz、8mT)へのばく露(28日間)による影響に対するハスの莢抽出成分プロシアニジン(LSPCs)の保護効果とそのメカニズムを検討した。その結果、LSPCsがマウスの臓器指数を上昇させ、ELF-EMFばく露により損傷された造血機能およびサイトカインレベルを見かけ上の通常レベルにまで回復させることが示されたとして、そのメカニズムについて議論している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The effects of exposure of mice to a 50 Hz magnetic field on the immune functions of the spleen and the protective effect of lotus seedpod procyanidins should be investigated.

詳細情報

Several studies showed that procyanidins have several biological functions, among others, they might enhance the function of the immune system.
Mice were divided into 5 groups (n=10 each): 1) exposure to the magnetic field and saline solution administration, 2) exposure to the magnetic field and administration of 30 mg/kg lotus seedpod procyanidins (LSPC) extract, 3) exposure to the magnetic field and administration of 60 mg/kg LSPC extract, 4) exposure to the magnetic field and administration of 90 mg/kg LSPC extract, 5) control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 4 h/day for 28 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 4 h/day for 28 days
ばく露装置
ばく露の発生源/構造
  • 詳細不明
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 8 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • 免疫
  • thymus, spleen, liver, kidney, spleen homogenates
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to the magnetic field (group 1) resulted in a significant reduction of relative weights of thymus, spleen and kidney compared to the control group. Mice with an additional administration of 90 mg/kg LSPCs (group 4), however, showed a significant increase of the organ weights compared to group 1.
The amounts of leukocytes, lymphocytes and hemoglobin were significantly reduced in group 1 compared to the control group, while they were significantly increased in group 4 compared to group 1.
The protein expressions of INF-gamma, IL-2, IL-6 and IL-10 were significantly reduced and the expression of TNF-alpha was significantly increased in group 1 compared to the control group. These effects were significantly reduced in groups 3 and 4.
The DNA content was significantly reduced in group 1 compared to the control group and it was significantly increased in group 4 compared to group 1.
The number of early and late apoptotic splenocytes was significantly reduced in group 1 compared to the control group and it was significantly increased in groups 3 and 4 compared to group 1.
In cell cycle analysis, group 1 had a significant larger G0 phase/G1 phase fraction and smaller S phase fraction compared to the control group. This effect was reduced by an additional LSPCs treatment (groups 2-4).
The magnetic field (group 1) significantly reduced Bcl-2 and Bcl-xl protein expression and significantly increased Bax, Caspase-3 and -9 levels. These effects were attenuated by LSPCs treatment and in group 4 there were significant differences in all expression levels compared to group 1.
The authors conclude that exposure of mice to a 50 Hz magnetic field might impair splenocytes via activation of Bax, caspases-3 and -9, and that lotus seedpod procyanidins could have a protective effect.

研究の種別:

研究助成

関連論文