研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界ばく露によりマウスに誘導された酸化ストレスに対するハスの莢抽出成分プロシアニジンの化学保護作用] med./bio.

Chemoprotective action of lotus seedpod procyanidins on oxidative stress in mice induced by extremely low-frequency electromagnetic field exposure.

掲載誌: Biomed Pharmacother 2016; 82: 640-648

この研究は、ICR雄マウス(n=60)を用いた実験で、超低周波電磁界(ELF-EMF:50Hz、8mT)へのばく露(28日間)の影響に対するハスの莢抽出成分プロシアニジン(LSPCs)の保護効果とそのメカニズムを検討した。マウスは無作為に同数の5群に分けた。対照群はLSPCsおよびELF-EMF無しで生理食塩水の経口投与のみ、ELF-EMFばく露の4群は、15日連続でLSPCs(体重1kg当たり60、90、120mg)、あるいは生理食塩水を毎日経口投与した後、4群とも28日間連続でELF-EMFばく露した。ばく露終了後に、血液および大脳皮質スーパーオキシドジスムターゼ(SOD)、カタラーゼ(CAT)、グルタチオン・ペルオキシダーゼGSH-Px)、グルタチオン還元酵素(GR)、グルタチオン-S-転移酵素(GST)、マロンジアルデヒドMDA)を分析した。その結果、異なる用量のLSPCs投与は、ELF-EMFによりマウスに誘導される酸化ストレス損傷を有意に阻止した;LSPCs投与は、SOD、CAT、GSH-Px、GR、GST活性を増加させ、MDAレベルを低下させた、と報告している。

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研究目的(著者による)

The effects of exposure of mice to a 50 Hz magnetic field on oxidative stress in the brain and serum and the protective effect of lotus seedpod procyanidins should be investigated.

詳細情報

In a previous study (Duan et al. 2013), the authors reported that lotus seedpod procyanidins (LSPCs) treatment prevented a magnetic field related increase in oxidation products and deficits in cognitive functions of mice, which suggested that LSPCs may have the potential to stimulate antioxidant enzyme activity and to scavenge oxygen free radicals.
First, the effect of different magnetic flux densities was investigated and male mice were divided into the following groups (n=12 each): exposure to a magnetic field of 1) 2 mT, 2) 4 mT, 3) 6 mT, 4) 8 mT, 5) 10 mT and 6) sham exposure for 7-28 days (4 h/day).
Secondly, the protective effect of LSPCs was investigated. LSPC extracts were administered orally once per day 15 days prior to and during exposure. Male mice were divided into the following groups (n=12 each): 7) exposure to an 8 mT magnetic field for 28 days (4 h/day) and oral saline administration, 8) exposure and 60 mg/kg LSPCs, 9) exposure + 90 mg/kg LSPCs, 10) exposure + 120 mg/kg LSPCs, 11) sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露2: 50 Hz
ばく露時間: continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露3: 50 Hz
ばく露時間: continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露4: 50 Hz
ばく露時間: continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露5: 50 Hz
ばく露時間: continuous for 7, 14, 21 and 28 days (4 h/day)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露装置
ばく露の発生源/構造
チャンバの詳細 ventilated perspex boxes of dimensions 14 cm x 10 cm x 12 cm in a room at a controlled temperature (23°C ± 2°C)
ばく露装置の詳細 the magnetic field was generated with a pair of Helmholtz coils (1.0 mm wire diameter, 32 cm internal diameter, 36 cm external diameter, 500 turns) placed vertically to the ground; mice were placed in the boxes in the middle of the pair of coils, but the box was not in contact with the coils; the temperature inside the ventilated perspex box was 23°C ± 0.5°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 4 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 6 mT - 測定値 - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 8 mT - 測定値 - -

ばく露5

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 and 28 days (4 h/day)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

28 days of exposure to an 8 mT (group 4) and 10 mT (group 5) field showed the most distinct significant decreases in SOD enzyme activity and most distinct significant increase in lipid peroxidation compared to the sham exposure. For energy saving and safety reasons, 8 mT for 28 days was chosen as exposure conditions for further tests with LSPCs.
Body weight was significantly decreased after exposure (group 7) compared to the sham exposure group (group 11) after 28 days of exposure. Additional treatment with 120 mg/kg LSPCs (group 10) significantly increased the body weight after 28 days of exposure compared to group 7.
After 28 days, the enzyme activities of all antioxidant enzymes in serum and cerebral cortex were significantly decreased in group 7 compared to the sham exposure group and they were significantly increased in group 10 compared to group 7. Likewise, lipid peroxidation was significantly increased in serum and cerebral cortex of group 7 compared to the sham exposure group and it was significantly decreased in group 10 compared to group 7 after 28 days.
In histological examinations, alterations and significant tissue damage were observed in cerebral cortex, thymus and spleen samples from group 7 after 28 days compared to the sham exposure group. These changes were attenuated in LSPC treatment groups (groups 8-10).
The authors conclude that exposure of mice to a 50 Hz magnetic field might induce oxidative stress in the brain and serum and that lotus seedpod procyanidins could have a protective effect.

研究の種別:

研究助成

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