研究のタイプ: 医学/生物学の研究 (experimental study)

50-Hz Sinusoidal Magnetic Field Effect On In Vitro Pineal N-Acetyltransferase Activity. med./bio.

掲載誌: Electro Magnetobiol 2000; 19 (3): 339-343

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研究目的(著者による)

The effects of exposure of rat pineal glands to a 50 Hz magnetic field on the enzyme activity of N-acetyltransferase should be investigated.

詳細情報

The background of the study was the question whether the pineal gland can be influenced directly by ELF MF or rather indirectly, e.g., via the retina. N-acetyltransferanse is necessary for the synthesis of melatonin in the pineal gland.
A total of 48 rats were sacrificed under a dim red light 6 h after the beginning of the dark period, which corresponds to the expected peak time of N-acetyltransferase enzyme activity. Their pineal glands were removed and transfered into test tubes. Afterwards the pineal glands were assigned to the following groups: exposure to a magnetic flux density of 1) 10 µT, 2) 100 µT 3) 1,000 µT or 4) sham exposure. Each of these groups contained 6 glands and further 6 glands were used as a separate control group for each of these groups, respectively.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 1 hour
ばく露2: 50 Hz
ばく露時間: 1 hour
ばく露3: 50 Hz
ばく露時間: 1 hour

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 1 hour
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 a circular solenoid of 425 turns in five layers (85 turns in each layer), 200 mm in diameter and 200 mm in height; the test tubes were introduced into a vessel filled with water inside the coil; the exposed glands and the controls were kept at 37 ± 0.5°C by a system of circulating water; no metallic element was used at a distance less than 2 m; the control groups were incubated in a similar system but without the coil, located 2 m from the exposure system
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 µT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 1 hour
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 1 hour
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1,000 µT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After exposure to a magnetic flux density of 1,000 µT (group 3), the N-acetyltransferase enzyme activity was significantly decreased compared to the control group.
All other exposure groups did not show any significant differences compared to the control groups.
The authors conclude that exposure of rat pineal glands to a 50 Hz magnetic field might affect the enzyme activity of N-acetyltransferase. This result supports the idea that the pineal gland can be directly affected by ELF MF.

研究の種別:

研究助成

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