研究のタイプ: 医学/生物学の研究 (experimental study)

[未熟及び成獣ラットに対する高周波電磁界(GSM1800 MHz)の細胞毒性及び遺伝毒性作用] med./bio.

Cytotoxic and genotoxic effects of high-frequency electromagnetic fields (GSM 1800MHz) on immature and mature rats.

掲載誌: Ecotoxicol Environ Saf 2012; 80: 140-144

この研究は、未熟及び成熟ラット骨髄細胞に対する、45日間の高周波電磁界ばく露(1800MHz、SARは0.37Wkgまたは0.49W/kg)の細胞毒性作用、及び電磁界ばく露後15日間の回復期間での変化を調べた。その結果、未熟・成熟、SARに拘わらず、全てのばく露理群において、染色体異常(CA)、小核MN)発生頻度、分裂指数(MI)及び多染性赤血球(PCEs)の割合に有意差が観察された;細胞毒性損傷は、未熟ラットでより顕著で、未熟ラットでは回復期間での改善は見られなかった、などの所見を報告している。

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研究目的(著者による)

To study the potential cytotoxic and genotoxic effects of long-term (45 days) exposure to 1800 MHz electromagnetic fields on bone marrow cells in immature and mature rats.

詳細情報

24 immature (two weeks old) and 24 mature (ten weeks old) rats were divided into six groups (each group n=8): 1) immature control group, 2) immature exposure group, 3) immature exposure group + recovery period of 15 days, 4) mature control group, 5) mature exposure group, 6) mature exposure group + recovery period of 15 days.
Bone marrow cells were collected from both femurs. Four rats were used for the micronucleus test and four rats for the chromosomal analysis and mitotic index.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for two hours/day for 45 days
  • SAR: 0.37 W/kg (immature rats)
  • SAR: 0.49 W/kg (mature rats)
  • 電界強度: 16.62 V/m mean (± 1,67 V/m (mature rats))
  • 電界強度: 16.96 V/m mean (± 5,39 V/m (immature rats))
  • 電力: 2 W maximum ((power of the GSM modulator))

General information

Rats were divided into six groups with eight animals each: i) immature control group ii) immature rats exposed to EMF iii) immature rats granted a recovery period of 15 days after exposure termination iv) mature control group v) mature rats exposed to EMF vi) mature rats granted a recovery period of 15 days after exposure termination

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
偏波
  • circular
ばく露時間 continuous for two hours/day for 45 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • モノポール
Distance between exposed object and exposure source 4 cm
チャンバの詳細 pie cage plexiglas restrainer with twelve slices: one rat/slice; numerous holes for air condition at the top of the restrainer
ばく露装置の詳細 antenna (height: 15 cm) located in the middle of the pie cage; antenna fixed about 4 cm from the head (due to movements of rats, the distance ranged from 4 to 8 cm)
Additional information band interval of GSM modulator: 1750 - 1850 MHz
パラメータ
測定量 種別 Method Mass 備考
SAR 0.37 W/kg - - whole body immature rats
SAR 0.49 W/kg - - whole body mature rats
電界強度 16.62 V/m mean 計算値 - ± 1,67 V/m (mature rats)
電界強度 16.96 V/m mean 計算値 - ± 5,39 V/m (immature rats)
電力 2 W maximum - - (power of the GSM modulator)

Reference articles

  • Aydin B et al. (2011): [ラットのリンパ器官、多形核白血球、血漿における酸化ストレスパラメータに対する900 MHz電磁界の影響]
  • Dasdag S et al. (2009): [ラットの脳におけるグリア細胞のアポトーシス及び酸化ストレス状態に対する携帯電話ばく露の影響]
  • Koyu A et al. (2005): [ラットのTSHおよび甲状腺ホルモンに対する900 MHz電磁界の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed significant differences in chromosome aberrations (increase), the micronucleus frequency (increase), mitotic index (decrease) and the ratio of polychromatic erythrocytes (decrease) in bone marrow cells of all exposure groups compared with the control group. Additionally, the cytotoxic and genotoxic damage was more remarkable in immature rats than in mature rats. The 15 days of recovery period was insufficient to compensate the genotoxic damage in immature rats after exposure.

研究の種別:

研究助成

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