研究のタイプ: 医学/生物学の研究 (experimental study)

[雄ラットの生殖系に対する電磁界の影響] med./bio.

Influence of electromagnetic fields on reproductive system of male rats.

掲載誌: Int J Radiat Biol 2013; 89 (3): 147-154

この研究は、Wister系雄ラット生殖系に対する10GHz電磁界の影響を調べた。70日齢雄ラットばく露群と擬似ばく露群(それぞれ6匹)に分け、ケージ上方のホーンアンテナから、1日2時間、45日間、0.21mW/cm2の電磁界照射した(SARは0.014W/kg)。ばく露終了後、血液および摘出した精巣を検査した。その結果、ばく露群で見られた影響は、精細管管腔の縮小、アポトーシス細胞出現、リンパ球小核形成、DNA鎖切断精巣の大きさ縮小を伴うテストステロンレベル低下などであったと報告している。

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研究目的(著者による)

To investigate the effects of a 10 GHz electromagnetic field on male rat's reproductive system.

詳細情報

70 days old rats were sham exposed (n=6) or exposed (n=6). After the end of the experiment, blood samples were collected for determining chromosome aberration, testosterone level and performing micronucleus test. Additionally, spermatozoa were collected for determining the caspase-3 activity and morphology, as well as performing a comet assay. The experiments were repeated thrice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10 GHz
ばく露時間: continuous for 2 h/day for 45 days

ばく露1

主たる特性
周波数 10 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h/day for 45 days
Modulation
Modulation type unspecified
ばく露装置
ばく露の発生源/構造
チャンバの詳細 rats placed in Plexiglas cages, ventilated with 1 cm diameter holes; the cages were pasted with anechoic material (top was not covered)
ばく露装置の詳細 exposure cage was placed vertically below the antenna, so that all animals were exposed homogeneously with the same power level
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.21 mW/cm² maximum 測定値 - -
SAR 0.014 W/kg maximum 計算値 whole body -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • testis
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The incidence of occurence of micronuclei increased statistically significant in the exposed group compared to the sham exposed group. No abnormalities in chromosomal number and structure was found in the exposed group in comparison to the sham exposed group. In the comet assay, a significant increase in tail length and intensity was shown in the exposed samples as compared to the sham exposed group. Sperm caspase-3 activity increased significantly in the exposure group, compared to the sham exposure group. The seminiferous tubules of the exposed group showed a significantly reduced diameter compared to them of the sham exposed groups. The serum testosterone level decreased significantly in exposed animals, as compared to sham exposed animals. The mean testicular weight of the exposed group was significantly reduced in comparison to the sham exposed group.
The authors conclude that the sperms of rats could be injured by 10 GHz electromagnetic fields.

研究の種別:

研究助成

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