研究のタイプ: 医学／生物学の研究 (experimental study)

[妊娠中の超高周波電磁界放射はラット子孫の赤血球での小核発生の増加をもたらす] med./bio.

Ultra high frequency-electromagnetic field irradiation during pregnancy leads to an increase in erythrocytes micronuclei incidence in rat offspring

著者: Ferreira AR, Knakievicz T, Pasquali MA, Gelain DP, Dal-Pizzol F, Fernandez CE, de Salles AA, Ferreira HB, Moreira JC
掲載誌: Life Sci 2006; 80 (1): 43-50
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この研究は、胚発生時に携帯電話からの非熱レベルの超高周波電磁界（UHF-EMF）のばく露を受けたラット子孫への影響を調べた。まず、赤血球における染色体損傷の発生について、哺乳動物のインビボ細胞遺伝学的試験である小核（MN）アッセイを用いて調べた。次に、末梢血および肝臓（ラットの胚および新生仔における重要な造血組織）の酸化パラメータの変化を調べるため、抗酸化酵素の活性、総スルフヒドリル含有量、タンパク質カルボニル基、チオバルビツール酸反応性種および非酵素的な総抗酸化防御能を測定した。その結果、UHF-EMFばく露群において、MN発生の有意な増加が見られた；ラットの子孫の血液と肝臓の酸化パラメータに有意差は見られなかった；同腹仔の平均の数にも、有意な変化はなかった、と報告している。

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研究目的（著者による）

This study should investigate in rats whether 1.) radiofrequency electromagnetic fields could induce chromosomal damage in erythocytes of rat offspring whose mothers were exposed during pregnancy and 2.) imbalances in free radical metabolism of rat offspring liver and blood occurs.

詳細情報

The exposure group consisted of six adult pregnant rats (90-110 days old, 200-250 grams) and the control group of four animals.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 834 MHz
ばく露時間: repeated daily exposure, 8.5 h/day, throughout pregnancy

ばく露1

主たる特性
周波数 834 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure, 8.5 h/day, throughout pregnancy
Additional information vertical polarisation
ばく露装置
ばく露の発生源／構造
  • セルラ－電話
  • analogue, in test mode, with stabilised power supply
Distance between exposed object and exposure source 29.5 cm
チャンバの詳細 Faraday cage (101.5 x 64 x 38 cm) made of 0.5-cm mesh
ばく露装置の詳細 The rats were housed in individual Plexiglas cages (40.5 x 33.5 x 17.5 cm) that were placed for exposure 29.5 cm away from the mobile phone antenna in its maximum radiation direction.
Sham exposure A sham exposure was conducted.
Additional information The Faraday cage containing the control animal was kept 40.5 cm away from the cage containing the exposure source. Both cages were placed in a wooden rack, and their places were switched every other day.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.6 W peak - - -
電界強度 40 V/m - 測定値 - 26.8-40 V/m
磁界強度 100 mA/m - 測定値 - 70-100 mA/m
電力密度 0.4 mW/cm² mean 測定値 - 0.2-0.4 mW/cm²
SAR 1.23 W/kg - 計算値 - 0.55-1.23 W/kg
Additional parameter details

The electric field measured inside the control cage did not differ from the background level (0.0-0.3 V/m).

測定および計算の詳細

The field intensity was measured using an RF spectrum analyser and EMF meters. The area corresponding to the rat cage was swept by the probe. The SAR was approximated by (σ/ρ)E²rms. The parameters for the rats were measured according to [Peyman et al., 2001].

Reference articles

  • Peyman A et al. (2001): [マイクロ波周波数でのラット組織の誘電特性の年齢の関数としての変化]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
  • blood, liver
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A statistically significant increase in micronuclei occurrence was observed in the irradiated group.
No significant differences were found in any oxidative parameter of offspring plasma and liver. Additionally, the average number of pups in each litter has not been significantly altered.
These data suggest that radiofrequency electromagnetic field is able to induce a genotoxic response in hematopoietic tissue during the embryogenesis. An imbalance in free radicals does not seem to play an important role in its genesis.

研究の種別:

研究助成

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