研究のタイプ: 医学／生物学の研究 (experimental study)

[900 MHz連続波電磁界とゲンタマイシンの重畳ばく露のラット聴覚に対する影響] med./bio.

Possible Combined Effects of 900 MHz Continuous-Wave Electromagnetic Fields and Gentamicin on the Auditory System of Rats

著者: Parazzini M, Galloni P, Piscitelli M, Pinto R, Lovisolo GA, Tognola G, Ravazzani P, Marino C
掲載誌: Radiat Res 2007; 167 (5): 600-605
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この研究は、900 MHzの電磁界へのばく露がSprague-Dawleyラットの蝸牛機能に与える影響を評価した。電磁界ばく露は、単独あるいはゲンタマイシンとの組み合わせばく露で実験を行い、蝸牛機能の評価には、蝸牛の外有毛細胞の状態を反映する指標である歪成分耳音響放射（DPOAE）を用いた。32匹のラットを4群に分けた。ゲンタマイシン投与群、ゲンタマイシン＋電磁界ばく露群、電磁界単独ばく露群、電磁界擬似ばく露群である。ゲンタマイシン投与は、150 mg / kg体重の筋肉内注射を15日間毎日行った。電磁界は、ラット耳での局所SARが4 W / kgの900 MHzの連続波で、2時間/日、5日/週、4週間ばく露した。ばく露の前、最中および後にDPOAE試験を実施した。その結果、正常な耳または既知の病理学的因子（ゲンタマイシン）ばく露を受けた耳のいずれにおいても、電磁界への亜慢性ばく露がラットの内耳聴覚機能に影響することは示されなかった、と報告している。

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研究目的（著者による）

To evaluate the cochlear functionality of rats exposed to electromagnetic fields at 900 MHz and to gentamicin (loss of hearing or balance is a well-documented adverse effect of such aminoglycoside antibiotics).

詳細情報

32 rats were divided into four groups: group 1 was treated with daily intramuscular injections of gentamicin for 15 days (150 mg/kg); group 2 was treated with daily intramuscular injections of gentamicin for 15 days (same dose) and exposed to electromagnetic fields; group 3 was exposed to electromagnetic fields; group 4 was sham-exposed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 2 h/day, 5 days/week, for 4 weeks

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure, 2 h/day, 5 days/week, for 4 weeks
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 10 mm
チャンバの詳細 The exposure system consisting of three distinct arrays of four loop antennas to expose 12 rats simultaneously was assembled in a wooden rack with RF absorbing panels on the four sides and between the levels [Galloni et al., 2005]. The arrays were used simultaneously, two for real and one for sham exposure, and were connected (blinded) to the MW generator through a black box.
ばく露装置の詳細 Rats were individually restrained in cylindrical Perspex tubes with a cone-shaped head end. The antenna was held in contact with the tube perpendicular to the head surface over the right ear of the rat at a total distance of about 8-10 mm from the cochlea (localised exposure).
Sham exposure A sham exposure was conducted.
Additional information Rats were placed close to a different antenna (in the same array) each day to ensure a uniform distribution of exposure. The antennas were rectangular loops printed on dielectric substrate using the coplanar line technique [Leveque et al., 2004].
パラメータ
測定量 値 種別 Method Mass 備考
SAR 4 W/kg - 測定値および計算値 - -
Additional parameter details

Since the rat could still make small movements, the SAR in the ear could vary (by less than 20%). Therefore, a periodic check of the rat's position was made by the operator to maintain the fixed exposure conditions.

測定および計算の詳細

Both numerical and experimental dosimetry were performed on the system and showed a good agreement (5.5 ± 1.0 W/kg and 4.42 ± 1.2 W/kg at 1 W input power) [Galloni et al., 2005]. The local SAR was measured at single points by inserting a fiberoptic probe in the cochlea of a rat's cadaver.

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
  • 感覚器
  • ear
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

The data showed no evidence of a combined effect of a radiofrequency electromagnetic field exposure and gentamicin on the auditory system of rats under these experimental conditions.

研究の種別:

研究助成

関連論文