研究のタイプ: 医学/生物学の研究 (experimental study)

[MCF10A細胞株の酸化ストレスに対する837および1950 MHzの無線周波放射の単一または組み合わせばく露の影響] med./bio.

Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells.

掲載誌: Bioelectromagnetics 2012; 33 (7): 604-611

この実験研究は、837MHz、1950MHzの無線周波放射のどちらかの単一周波数ばく露または両者の組み合わせばく露がヒトMCF10A細胞株酸化ストレスを引き起こすか否かを調べた。それぞれの単一ばく露および組み合わせばく露は全て、SAR値4 W/kg、ばく露時間2時間で行った。ばく露中温度上昇はないことを確認した。その結果、細胞内活性酸素種レベル、スーパーオキシドディスムターゼの抗酸化酵素活性還元型/酸化グルタチオン比とも、単一および組み合わせばく露のいずれにおいても統計学的に有意な変化が見られなかったと報告している。

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研究目的(著者による)

To investigate whether the exposure to either single or combined radiofrequency fields could induce oxidative stress in human breast epithelium cells.

詳細情報

The following groups were investigated:
1.) sham exposure,
2.) exposure to CDMA signal at 837 MHz,
3.) exposure to W-CDMA signal at 1950 MHz,
4.) combined exposure to CDMA signal at 837 MHz and W-CDMA signal at 1950 MHz and
5.) positive control: 3 Gy gamma radiation.
Cells were investigated at 10, 24 and 48 h following exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 837 MHz
ばく露時間: continuous for 2 h
ばく露2: 1,950 MHz
ばく露時間: continuous for 2 h
ばく露3: 837–1,950 MHz
ばく露時間: continuous for 2 h

ばく露1

主たる特性
周波数 837 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Additional information CDMA
ばく露装置
ばく露の発生源/構造
  • conical antenna
Distance between exposed object and exposure source 13.6 cm
ばく露装置の詳細 cells placed in 100 mm Petri dishes, 3 - 6 dishes positioned around the antenna, which was placed in the center of the exposure chamber
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - - -

ばく露2

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Additional information W-CDMA
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between exposed object and exposure source 13.6 cm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - - -

ばく露3

主たる特性
周波数 837–1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Additional information 837 MHz CDMA signal + 1950 MHz W-CDMA signal
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Distance between exposed object and exposure source 13.6 cm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - CDMA
SAR 2 W/kg - - - W-CDMA

Reference articles

  • Lee KY et al. (2011): [細胞周期とその調節タンパク質に対する無線周波放射への組合せばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Radiofrequency field exposed groups did not show any changes in cell morphology and growth compared to the sham exposed group.
Additionally, there were no changes in the activity of superoxide dismutase, in the level of glutathione/glutathione disulfide and in the level of reactive oxygen species in exposed cells in comparison to sham exposed cells.
In contrast, ionizing radiation exposed cells showed evident changes in all measured biological endpoints.
The authors conclude that neither single nor combined exposures to a radiofrequency field elicit oxidative stress in human breast epithelium cells under the examined exposure conditions.

研究の種別:

研究助成

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