研究のタイプ: 医学/生物学の研究 (experimental study)

[ジアシルグリセロール・アシルトランスフェラーゼのmRNA発現レベルおよびトリアシルグリセロール、遊離脂肪酸、リン脂質および総コレステロールの血漿濃度における50Hz電界の影響] med./bio.

Effect of 50 Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol.

掲載誌: Lipids Health Dis 2012; 11: 68-

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研究目的(著者による)

To study the effects of exposure to a 50 Hz electric field on plasma level of triacylglycerol, free fatty acids, total cholesterol and phospholipid and mRNA expression of diacylglycerol acyltransferase genes (which encode enzymes involved in the synthesis of triacylglycerol ) in liver and intestines from mice.

詳細情報

Six mice were exposed and six mice were sham exposed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 30 min/day for 11 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
ばく露時間 continuous for 30 min/day for 11 days
ばく露装置
ばく露の発生源/構造
  • stainless steel electrodes
ばく露装置の詳細 20 cm high cylindrical plastic cage with a diameter of 20 cm (with 10 cm long and 0,5 cm wide slits at 5 mm intervals); 1m x 60 cm stainless steel electrodes on top and at the bottom of the cage
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 45 kV/m effective value 測定値 - -

Reference articles

  • Harakawa S et al. (2008): [条件付き嫌悪応答における電界の影響]
  • Harakawa S et al. (2005): [下肢虚血ラットにおける乳酸ならびにグルコース,遊離脂肪酸,トリグリセリド,クレアチンホスホキナーゼの血中レベルに対する50 Hz電界ばく露の影響]
  • Hori T et al. (2005): [C57BL/6J雄マウスの電界へのばく露は過剰排卵する雌マウスとの交尾率を改善する]
  • Harakawa S et al. (2005): [ラットの血漿脂質過酸化レベル及び抗酸化活性に対する50Hz電界の影響]
  • Harakawa S et al. (2004): [ストレスを受けたラットの血漿ACTH、グルコース、乳酸、ピルビン酸レベルへの50 Hz電界の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • liver and intestine
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The diacylglycerol-o-acyltransferase gene 2 (DGAT2) expression in the liver of exposed mice was significantly lower than that of the control. Additionally, both plasma total cholesterol and phospholipid in the exposed mice were lower than those in the control. However, there was no difference in DGAT1 expression, triacylglycerol or free fatty acid levels.
The mechanisms for the effects of electric fields on lipid metabolism are not well understood yet, but altered DGAT2 activity may be involved.

研究の種別:

研究助成

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