研究のタイプ: 医学/生物学の研究 (experimental study)

[長期観察における電磁界が誘導した細胞増殖の逆転現象] med./bio.

Electromagnetic field-induced converse cell growth during a long-term observation.

掲載誌: Int J Radiat Biol 2013; 89 (12): 1035-1044

この研究は、ヒトがん細胞培養株(DU145およびJurkat)の増殖に対する電力周波磁界MF;60 Hz, 1 mT)への3日間のばく露培養器内に設置したヘルムホルツコイルによる)の影響をばく露終了後長期にわたり観察した。3日間のばく露後、DU145細胞株は5.3年間、Jurkat細胞株は2.3年間、擬似ばく露群および対照群と共に継続的に観察し、観察期間中に随時、2-(4-Iodophenyl)- 3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium(WST-1)試験およびトリパンブルー色素排除試験を実施した。そのようにして得た増殖力のペリオドグラムを周期解析した。その結果、2種類の細胞株増殖に対するMFの影響は、周期性をもって促進的または抑制的になった;MFが誘導した細胞増殖のこのような逆転現象は、同時に実験した3つの培養器間で一貫していた、と報告している。

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研究目的(著者による)

To examine the effect of magnetic field exposure on the cell proliferation of cancer cells.

詳細情報

In a previous study of the authors (Koh et al., 2008), an inhibitory effect of exposure to a 60 Hz magnetic field on the cell proliferation of cancer cells was observed. The present study was originally designed to analyze the molecular mechanisms of this inhibitory effect. However, the previous and the present study showed contradictory results. Therefore, the effect of magnetic field-exposure on the cell proliferation of cancer cells was observed over a long time period. During 5 years, experiments were consistently repeated. Hereby, the cell cultures were started from the same cryoculture, respectively. Every experiment started with a 24 hours pre-incubation step. Afterwards, cells were treated with one of the following conditions for three days: 1.) not exposed, 2.) sham exposed or 3.) exposed. For sham exposure and exposure, three different incubators were used. The experiments were conducted in duplicates.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 3 days

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 3 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 Helmholtz coils were comprised of 3 rectangular bundles (2,000-turns per bundle, 0.5 mm diameter of copper wire) on open acrylic frames, aligned in parallel 20 cm apart (28 Œ 28 Œ 30 cm); for electromagnetic field exposure, electric current passed through Helmholtz coils, was generated by rheostat connected to a voltage transformer
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

Reference articles

  • Koh EK et al. (2008): [60Hz正弦波磁界は活性酸素種を介して前立腺がん細胞のアポトーシスを誘導する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Both cell types, independently if exposed or sham exposed, showed positive and negative differences in cell proliferation throughout the years compared to the control cell cultures. These effects occurred in a period-dependent manner and differed between the used incubators.
The authors conclude that the observed spatiotemporal differences in the present study may partially explain the poor reproducibility and controversial results of many experimental and epidemiological studies.

研究の種別:

研究助成

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