この研究は、ヒトがん細胞培養株（DU145およびJurkat）の増殖に対する電力周波磁界（MF；60 Hz, 1 mT）への3日間のばく露（培養器内に設置したヘルムホルツコイルによる）の影響をばく露終了後長期にわたり観察した。3日間のばく露後、DU145細胞株は5.3年間、Jurkat細胞株は2.3年間、擬似ばく露群および対照群と共に継続的に観察し、観察期間中に随時、2-(4-Iodophenyl)- 3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium（WST-1）試験およびトリパンブルー色素排除試験を実施した。そのようにして得た増殖力のペリオドグラムを周期解析した。その結果、2種類の細胞株の増殖に対するMFの影響は、周期性をもって促進的または抑制的になった；MFが誘導した細胞増殖のこのような逆転現象は、同時に実験した3つの培養器間で一貫していた､と報告している。
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To examine the effect of magnetic field exposure on the cell proliferation of cancer cells.
In a previous study of the authors (Koh et al., 2008), an inhibitory effect of exposure to a 60 Hz magnetic field on the cell proliferation of cancer cells was observed. The present study was originally designed to analyze the molecular mechanisms of this inhibitory effect. However, the previous and the present study showed contradictory results. Therefore, the effect of magnetic field-exposure on the cell proliferation of cancer cells was observed over a long time period. During 5 years, experiments were consistently repeated. Hereby, the cell cultures were started from the same cryoculture, respectively. Every experiment started with a 24 hours pre-incubation step. Afterwards, cells were treated with one of the following conditions for three days: 1.) not exposed, 2.) sham exposed or 3.) exposed. For sham exposure and exposure, three different incubators were used. The experiments were conducted in duplicates.
ばく露時間: continuous for 3 days
|ばく露時間||continuous for 3 days|
|ばく露装置の詳細||Helmholtz coils were comprised of 3 rectangular bundles (2,000-turns per bundle, 0.5 mm diameter of copper wire) on open acrylic frames, aligned in parallel 20 cm apart (28 Œ 28 Œ 30 cm); for electromagnetic field exposure, electric current passed through Helmholtz coils, was generated by rheostat connected to a voltage transformer|
|Sham exposure||A sham exposure was conducted.|
Both cell types, independently if exposed or sham exposed, showed positive and negative differences in cell proliferation throughout the years compared to the control cell cultures. These effects occurred in a period-dependent manner and differed between the used incubators.
The authors conclude that the observed spatiotemporal differences in the present study may partially explain the poor reproducibility and controversial results of many experimental and epidemiological studies.