研究のタイプ: 医学/生物学の研究 (experimental study)

[872 MHzの高周波放射線、酸化剤にばく露した細胞における増殖、酸化ストレスと細胞死] med./bio.

Proliferation, Oxidative Stress and Cell Death in Cells Exposed to 872 MHz Radiofrequency Radiation and Oxidants.

掲載誌: Radiat Res 2008; 170 (2): 235-243

【目的】ヒト神経芽細胞腫細胞SH-SY5Yおよびマウス線維芽細胞L929を、SAR値5W/kgの非熱的条件で、872MHzのRF電磁界連続波またはGSM携帯電話放射に似せた変調波)にばく露させ、その他の因子との複合影響を調べること。方法:メナジオン(活性酸素種誘導させるため)、およびtert-ブチルヒドロオキシド(t-BOOH:脂質過酸化誘導させるため)を用いた。1時間または24時間のばく露を行った後、細胞グルタチオンレベル低下、脂質過酸化増殖率、カスパーゼ3活性、DNA断片化、細胞生存率を測定した。結果:RF電磁界に関連した統計学的に有意な差異は、t-BOOH誘導による脂質過酸化がSH-SY5Y細胞で増加したこと(L929では見られなかった)、メナジオン誘導によるカスパーゼ3活性がL929細胞で増加したこと(SH-SY5Yでは見られなかった)の2点であった。この2点はGSM変調信号に対してのみ統計学的に有意であった。その他の影響については全ての実験条件で大きな変化はなく、またRFばく露単独の場合にも影響はなかった。

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研究目的(著者による)

To investigate the effects of pulsed wave, GSM modulated or continuous wave 872 MHz radiofrequency irradiation on cell proliferation, apoptosis, other forms of cell death, and oxidative stress.

詳細情報

To examine possible combined effects of radiofrequency exposure with other agents, cells were treated with menadione to induce reactive oxygen species, and with tertiary butylhydro-peroxide to induce lipid peroxidation. The experiments were performed isothermal and with a specific absorption rate of 5 W/kg which is obviously above the ICNIRP limit value.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 872 MHz
Modulation type: CW
ばく露時間: continuous for 1 or 24 h
ばく露2: 872 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 or 24 h

General information

A comprehensive description of the exposure system including details about the dosimetry has been published in [Hoyto et al., 2006].

ばく露1

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1 or 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 CO2 (5%) and temperature-controlled air were provided to the aluminium RF resonator by ventilation from a cell culture incubator. Temperature increases in the cell cultures were eliminated by placing the culture dishes on a heat exchanger with water circulation, which ensured isothermal conditions (37.0 ± 0.3°C).
ばく露装置の詳細 Cells in two 55-mm glass or plastic Petri dishes were RF exposed in one chamber, and two dishes were simultaneously sham-exposed in an identical chamber.
Sham exposure A sham exposure was conducted.
Additional information The cells were subjected to four conditions: no exposure, RF radiation, chemical treatment, and RF radiation plus chemical treatment.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

ばく露2

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1 or 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM-like

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

Reference articles

  • Höytö A et al. (2006): [適度の温度上昇はL929細胞のODC活性に影響する:低レベルのラジオ周波数では影響は見られない]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The lipid peroxidation induced by tertiary butylhydro-peroxide was statistically significant increased in radio frequency exposed human neuroblastoma cells, but not in radio frequency exposed mouse fibroblasts. Additionally, menadione-induced caspase 3 activity was statistically significant increased in radio frequency exposed mouse fibroblasts, but not in radio frequency exposed human neuroblastoma cells. Both differences were statistically significant only for the GSM-modulated signal. The other endpoints were not significantly affected in any of the experimental conditions, and no effects were observed from exposure to radio frequency irradiation alone.
The authors conclude that the positive findings may be due to chance, but they may also reflect effects that occur only in cells sensitized by chemical stress. Further studies are required to investigate the reproducibility and dose response of the possible effects.

研究の種別:

研究助成

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