研究のタイプ: 医学/生物学の研究 (experimental study)

[雌の仔ラットの脊髄の形態および運動行動に対する出生前の900メガヘルツ電磁界へのばく露の影響] med./bio.

The Effects of 900 Megahertz Electromagnetic Field Applied in the Prenatal Period on Spinal Cord Morphology and Motor Behavior in Female Rat Pups.

掲載誌: Neuroquantology 2013; 11 (4): 573-581

この研究は、雌の仔ラット脊髄および運動行動に対する900MHz電磁界への出生前ばく露の影響を調査した。妊娠した雌のSprague-Dawleyラット対照群およびばく露群に分けた(各n=3)。ばく露群は900MHz電磁界に1時間/日、妊娠13日目から21日目までばく露した。21日齢の時点で、仔ラットを母親から離し、対照群(n=13)およびばく露群(n=10)に分けた。仔ラットの運動機能をロータロッド試験で、自発運動活性をオープンフィールド試験で評価した。生後32日目に仔ラットを断頭し、上部胸郭から脊髄を摘出し、通常の組織学的検査を実施後、クレシルファストバイオレットで染色した。ロータロッド試験の結果、ばく露群の仔ラットの運動機能に有意な増加(p=0.037)が認められたが、オープンフィールド試験では差は認められなかった(p>0.05)。ばく露群の仔ラットでは、脊髄組織学的変化が認められた。著者らはこの結果から、900MHz電磁界への出生前ばく露脊髄形態学的変化の形で脊髄の発達に影響を及ぼし、これは仔ラットの自発運動活性の上昇につながるかも知れない、と結論付けている。

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研究目的(著者による)

The effects of prenatal exposure of female rats to a 900 MHz electromagnetic field on spinal cord morphology and motor activity in young rats should be investigated.

詳細情報

Pregnant rats were either exposed or not exposed (n=3, respectively). When rat pups were 21 days old, female pups from non-exposed mothers were assigned to the control group (n=13) and pups from exposed mothers were assigned to the exposure group (n=10). At the age of 26 days, motor activity tests were conducted and at the age of 32 days, rats were killed and histological examinations were conducted.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: intermittent for 1 hour/day from day 13 to 21 of pregnancy

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 1 hour/day from day 13 to 21 of pregnancy
ばく露装置
ばく露の発生源/構造
チャンバの詳細 plexiglass jar of 30 cm x 42 cm x 52 cm
ばく露装置の詳細 half-wave dipole antenna made from a copper rod (1 mm x 15 cm) was inserted into the central area of the jar, approximately 11 cm inside the open surface of the jar
Additional information exposure and control groups were maintained in separate cages in the same room during the experiment, except during exposure to EMF, which took place in the EMF exposure room
パラメータ
測定量 種別 Method Mass 備考
電力 300 mW - - - approximate output power
電界強度 10 V/m mean 計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Motor coordination and balance were significantly increased in exposed rats compared to the control group, while motor activity did not show any significant differences between the groups.
No pathologic changes were found in spinal cord samples from control rats, while exposed rats showed atrophy, vacuolization in gray matter and occasional myelin thickening, white matter infiltration among the nerve fibers and infiltration of gray matter inside white matter as well as structural impairment in motor neuron cell body structure.
The authors conclude that prenatal exposure of female rats to a 900 MHz electromagnetic field might affect spinal cord morphology which might cause a change in motor functions in young rats.

研究の種別:

研究助成

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