研究のタイプ: 医学/生物学の研究 (experimental study)

[ラット卵巣における周波数50Hzの非電離電磁界の影響:透過電子顕微鏡による研究] med./bio.

The effect of Non-ionizing electromagnetic field with a frequency of 50 Hz in Rat ovary: A transmission electron microscopy study.

掲載誌: Int J Reprod Biomed (Yazd) 2016; 14 (2): 125-132

この研究は、ラットに50Hz磁界ばく露を2通りのばく露期間で与え、ばく露終了後に卵巣を摘出し、卵胞および顆粒膜細胞の標本を電子顕微鏡で観察した。ばく露は、8週間ばく露子宮内で3週間、出生後5週間)、13週間ばく露子宮内で3週間、出生後10週間)である。2つのばく露群および対照群はそれぞれn=10であった。ばく露には直径20cmのヘルムホルツコイルを用いた。その結果、対照群では異常が観察されなかった;ばく露群では、一次卵胞卵胞膜が分離するなど形態学的変化が観察された、と報告している。(JEIC注:一定数の雌新生仔を得たプロセスや母ラットに関する説明、動物ばく露方法の説明は論文中にない。結果も顕微鏡像観察の記述のみである。)

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的(著者による)

The effects of prenatal and postnatal exposure of rats to a 50 Hz magnetic field on the ultrastructure of ovarian follicles should be investigated.

詳細情報

30 three-month old female rats were divided into 3 groups (n=10 each): 1) exposure to the magnetic field for 8 weeks (3 weeks prenatal and 5 weeks postnatal exposure), 2) exposure to the magnetic field for 13 weeks (3 weeks prenatal and 10 weeks postnatal exposure), 3) untreated control group.
(remark EMF-Portal: The experimental design is not clear. The three-month old rats were probably pregnant and their offspring was exposed and investigated.)

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50–60 Hz
ばく露時間: for 8 weeks (3 weeks prenatal and 5 weeks postnatal)
-
ばく露2: 50–60 Hz
ばく露時間: for 13 weeks (3 weeks prenatal and 10 weeks postnatal)
-

ばく露1

主たる特性
周波数 50–60 Hz
タイプ
  • magnetic field
ばく露時間 for 8 weeks (3 weeks prenatal and 5 weeks postnatal)
Additional information 50 or 50-60 Hz magnetic field? (contradictory specifications)
ばく露装置
ばく露の発生源/構造
チャンバの詳細 incubator
ばく露装置の詳細 the Helmholtz coils had a diameter of 20 cm and contained 200 windings of 0.8 mm copper wire located at a distance of 10 cm from each other
パラメータ

No parameters are specified for this exposure.

ばく露2

主たる特性
周波数 50–60 Hz
タイプ
  • magnetic field
ばく露時間 for 13 weeks (3 weeks prenatal and 10 weeks postnatal)
Additional information 50 or 50-60 Hz magnetic field? (contradictory specifications)
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ

No parameters are specified for this exposure.

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The control group showed cytoplasmic projections in zona pellucida, normal nucleus membrane and round or ovally shaped mitochondria.
In exposure group 1 (8 weeks of exposure), the nuclei of theca cells showed different proportions of heterochromatin, a sign of degeneration, and vacuolization was observed. In both exposure groups, the mitochondrial structure was changed and lipid droplets were visible in follicular cells.
In exposure group 2 (13 weeks of exposure), the nuclei of endothelial cells showed heterochromatin and were swollen. In cells from the granulosa layer, nuclei were irregular and dark.
The authors conclude that prenatal and postnatal exposure of rats to a 50 Hz magnetic field might have harmful effects on ovarian follicles.

研究の種別:

研究助成

関連論文