Study type: Medical/biological study (experimental study)

The effect of melatonin on the liver of rats exposed to microwave radiation. med./bio.

Published in: Bratisl Lek Listy 2015; 116 (2): 96-100

Aim of study (acc. to author)

The effects of exposure of rats to a 900 MHz electromagnetic field and co-exposure to melatonin on oxidative stress in the liver should be investigated.

Background/further details

84 rats were divided into 4 groups (n=21 each): 1) control group, 2) administration of melatonin, 3) exposure to microwaves, 4) administration of melatonin and exposure to microwaves. Seven animals of each group were sacrificed and examined after 20, 40 and 60 days of exposure, respectively.
2 mg/kg body weight melatonin was administered intraperitoneally every morning in groups 2 and 4. Animals of the control group received an equal volume of isotonic saline solution.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 4h/day for 20, 40 or 60 days

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Charakteristic
Exposure duration continuous for 4h/day for 20, 40 or 60 days
Additional info GSM frequency band-mobile transmitting signal
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber plastic cage without metallic fittings
Setup rats placed in plastic cage containing a mobile phone (Nokia 3110) with antenna positioned downwards; animals were able to move freely during exposure
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 4.86 µT minimum measured - according to abstract 100 µT (?)
magnetic flux density 8.69 µT maximum measured - according to abstract 300 µT (?)
electric field strength 9.88 V/m minimum measured - according to abstract 54 V/m (?)
electric field strength 18.356 V/m maximum measured - according to abstract 160 V/m (?)
SAR 0.089 W/g mean estimated whole body -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The lipid peroxidation was significantly increased in group 3 (exposure to microwaves) compared to the control group after 40 and 60 days of exposure. At the same time, it was significantly reduced in group 4 (melatonin + microwaves) compared to group 3.
Moreover, protein oxidation was significantly increased after 20, 40 and 60 days, catalase and xanthine oxidase enzyme activity were significantly increased after 40 and 60 days and the enzyme activity of alkaline deoxyribonuclease I was significantly increased after 60 days of exposure in group 3 compared to the control group and group 2 (melatonin only).
The authors conclude that exposure of rats to a 900 MHz electromagnetic field could cause oxidative stress in the liver and that melatonin might have an antioxidant effect by reducing lipid peroxidation.

Study character:

Study funded by

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