研究のタイプ: 医学/生物学の研究 (experimental study)

[異なる年齢の若いラットの肝臓における酸化的損傷、不飽和脂肪酸および坑酸化物質の代謝に対する950 MHzのUHF電磁界放射の影響] med./bio.

Effect of 950 MHz UHF Electromagnetic radiation on biomarkers of oxidative damage, metabolism of UFA and antioxidants in the liver of young rats of different ages.

掲載誌: Int J Radiat Biol 2014; 90 (2): 159-168

この研究は、ラットにおける酸化損傷のバイオマーカに対する超高周波電磁界放射UHF EMR;950MHz)の影響を調べると同時に、肝臓の不飽和脂肪酸(UFA)濃度とカタラーゼ発現を確認した。妊娠したドブネズミ(Rattus norvegicus)をそれぞれ対照群ばく露群(各群6匹)に分け、ばく露群は、出産までの21日間、SAR 1.3 - 1.0 W/kgに1日当たり1時間30分ばく露した。対照群ばく露群から生まれた仔ラットを0、6、15、30日齢でそれぞれ6匹ずつ屠殺し、脂質、タンパク、DNAの損傷を、それぞれチオバルビツール酸反応性物質(TBARS)、カルボニル化タンパク、コメットアッセイで測定した。またそれぞれの日齢でのUFA濃度とカタラーゼ発現を測定した。ばく露群から生まれた仔ラットは同条件のばく露を継続したため、例えば30日齢ラットは51日間ばく露を受けたことになる。その結果、TBARSレベルおよびUFA濃度は、ばく露した後の0日齢で低かった;カルボニル化タンパクの蓄積量は、どの日齢でも、対照群ばく露群で有意差がなかった;DNA損傷は、15、30日齢で、対照群ばく露群で差が見られた;カタラーゼ発現は、ばく露した後の0日齢で低かった、と報告している。

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研究目的(著者による)

To investigate the effects of an exposure to a 950 MHz electromagnetic field on biochemical processes in the liver of young rats.

詳細情報

During pregnancy, dams were exposed (n=6) or sham exposed (n=6). From each group, 6 pups were sacrificed in the age of 0, 6, 15 and 30 days, respectively and examined. In the age of 15 and 30 days, only male rats were examined.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 950 MHz
ばく露時間: 30 minutes/day for 21 days during pregnancy
neonatal rats
  • SAR: 0.88 W/kg (for neonatal rats)
  • SAR: 0.01 W/kg minimum (for pregnant rats)
  • SAR: 0.03 W/kg maximum (for pregnant rats)
  • 電界強度: 28.9 V/m cf. remarks (internal value for liver for neonatal rats)
  • 電界強度: 35 V/m mean (in the middle of the cage)
ばく露2: 950 MHz
ばく露時間: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 6 days
6 day-old rats
  • SAR: 0.51 W/kg (for 6 day-old rats)
  • SAR: 0.01 W/kg minimum (for pregnant rats)
  • SAR: 0.03 W/kg maximum (for pregnant rats)
  • 電界強度: 22 V/m cf. remarks (internal value for liver for 6 day-old rats)
  • 電界強度: 35 V/m mean (in the middle of the cage)
ばく露3: 950 MHz
ばく露時間: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 15 days
15 day-old rats
  • SAR: 0.18 W/kg (for 15 day-old rats)
  • SAR: 0.01 W/kg minimum (for pregnant rats)
  • SAR: 0.03 W/kg maximum (for pregnant rats)
  • 電界強度: 15.7 V/m cf. remarks (internal value for liver for 15 day-old rats)
  • 電界強度: 35 V/m mean (in the middle of the cage)
ばく露4: 950 MHz
ばく露時間: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 30 days
30 day-old rats
  • SAR: 0.06 W/kg (for 30 day-old rats)
  • SAR: 0.01 W/kg minimum (for pregnant rats)
  • SAR: 0.03 W/kg maximum (for pregnant rats)
  • 電界強度: 8.5 V/m cf. remarks (internal value for liver for 30 day-old rats)
  • 電界強度: 35 V/m mean (in the middle of the cage)

ばく露1

主たる特性
周波数 950 MHz
タイプ
  • electromagnetic field
ばく露時間 30 minutes/day for 21 days during pregnancy
Additional information neonatal rats
ばく露装置
ばく露の発生源/構造
  • Half-wave dipole antenna
Distance between exposed object and exposure source 11 cm
ばく露装置の詳細 cages were placed inside an aluminium Faraday cage (101.5 x 64.0 x 38 cm); antenna was configured for vertical polarization
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.88 W/kg - 計算値 - for neonatal rats
SAR 0.01 W/kg minimum 計算値 - for pregnant rats
SAR 0.03 W/kg maximum 計算値 - for pregnant rats
電界強度 28.9 V/m cf. remarks 計算値 - internal value for liver for neonatal rats
電界強度 35 V/m mean 測定値 - in the middle of the cage

ばく露2

主たる特性
周波数 950 MHz
タイプ
  • electromagnetic field
ばく露時間 30 minutes/day for 21 days during pregnancy and postnatal until an age of 6 days
Additional information 6 day-old rats
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 0.51 W/kg - 計算値 - for 6 day-old rats
SAR 0.01 W/kg minimum 計算値 - for pregnant rats
SAR 0.03 W/kg maximum 計算値 - for pregnant rats
電界強度 22 V/m cf. remarks 計算値 - internal value for liver for 6 day-old rats
電界強度 35 V/m mean 測定値 - in the middle of the cage

ばく露3

主たる特性
周波数 950 MHz
タイプ
  • electromagnetic field
ばく露時間 30 minutes/day for 21 days during pregnancy and postnatal until an age of 15 days
Additional information 15 day-old rats
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 0.18 W/kg - 計算値 - for 15 day-old rats
SAR 0.01 W/kg minimum 計算値 - for pregnant rats
SAR 0.03 W/kg maximum 計算値 - for pregnant rats
電界強度 15.7 V/m cf. remarks 計算値 - internal value for liver for 15 day-old rats
電界強度 35 V/m mean 測定値 - in the middle of the cage

ばく露4

主たる特性
周波数 950 MHz
タイプ
  • electromagnetic field
ばく露時間 30 minutes/day for 21 days during pregnancy and postnatal until an age of 30 days
Additional information 30 day-old rats
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 0.06 W/kg - 計算値 - for 30 day-old rats
SAR 0.01 W/kg minimum 計算値 - for pregnant rats
SAR 0.03 W/kg maximum 計算値 - for pregnant rats
電界強度 8.5 V/m cf. remarks 計算値 - internal value for liver for 30 day-old rats
電界強度 35 V/m mean 測定値 - in the middle of the cage

Reference articles

  • Vecchia P et al. (2009): [高周波電磁界へのばく露、生物学的影響および健康への影響(100 kHz - 300 GHz)]
  • Peyman A et al. (2001): [マイクロ波周波数での年齢の関数としてのラット組織の誘電特性の変化]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Neonatal rats exposed in utero had a significanty decreased body weight, significantly decreased levels of catalase and significant lower lipid peroxidation values compared to neonatal rats from the control group. No significant differences in the body weight, the levels of catalase and the lipid peroxidation were observed after 6, 15 or 30 days between the groups.
No differences occurred in the level of protein oxidation between exposed and sham exposed rats.
The level of fatty acids was significantly decreased in exposed rats in comparison to sham exposed rats (Remark EMF-Portal: information about age of rats not given in this context).
15 and 30 day-old rats showed significant differences regarding DNA damage. In the age of 15 days, in the exposed rats, the number of DNA strand breaks was significantly lower compared to the control group, while it was significantly higher in the age of 30 days.
The data indicate that exposure to 950 MHz electromagnetic fields could have induced some biochemical changes in the liver of young rats.

研究の種別:

研究助成

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