The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
To study whether 24 h exposure to radiofrequency electromagnetic fields similar to those emitted by mobile phones induces genotoxic effects and/or effects on cell cycle kinetics in cultured human peripheral blood lymphocytes.
Genotoxic effects were evaluated in lymphocyte cultures from 10 healthy donors. Positive controls were provided by using mitomycin C (0.033 µg/ml).
Two research groups were involved in the study: one at ENEA, Rome, and the other at CNR-IREA, Naples. Each laboratory tested five donors, and the resulting slides were scored by both laboratories.
|ばく露時間||continuous for 24 h|
|Duty cycle||12.5 %|
|Repetition frequency||217 Hz|
|チャンバの詳細||The exposure system consisted of four WPCs, each fed by a different power level in a blinded procedure. Two WPCs shielded by metal grid boxes were placed in each of two incubators (5% CO2 and 95% air). Each WPC or at least each incubator had a dummy culture Petri dish for temperature measurement.|
|ばく露装置の詳細||The WPC [Laval et al., 2000] consisted of two parallel plates (15 x 15 x 2.9 cm³), short-circuited at the edges by four posts. The outer conductor of the RF supply coaxial cable was connected to the top plate, and the inner conductor was connected to the ground plane. The temperature of the exposed samples was maintained at 36.9 ± 0.5°C by spiral coils with circulating water positioned on each plate. Four 35-mm Petri dishes containing 3 ml of diluted blood were placed inside the cell where the E-field distribution was quite uniform.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Six conditions were tested in duplicate: RF exposure at peak SARs of 0 (sham), 1, 5 and 10 W/kg, a positive (MMC-treated) control, and a negative (unexposed and untreated) control. Positive and negative controls were kept for 24 h in a third CO2 incubator at 37°C.|
The data revealed no genotoxic or cytotoxic effects in the range of SAR values investigated. These findings were confirmed by two reserach groups and when the same slides were scored by two operators.