研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト神経芽細胞腫細胞SH-SY5Yの活性酸素種産生およびDNA損傷に対する872 MHz無線周波電磁界と塩化第一鉄の組合わせ影響] med./bio.

Combined effects of 872 MHz radiofrequency radiation and ferrous chloride on reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells.

掲載誌: Bioelectromagnetics 2010; 31 (6): 417-424

この研究は、ヒト神経芽細胞腫細胞SH-SY5Yの活性酸素種産生およびDNA損傷に対する872MHz無線周波RF電磁界と塩化第一鉄(FeCl2)の組合わせ影響を調べた。DNA損傷の基礎メカニズムとしての細胞内ROS産生の試験には蛍光プローブDCFH-DAを利用した。DNAの完全性はアルカリコメットアッセイで定量化した。872MHzの連続波(CW)またはGSM類似信号で、比吸収率5 W/kgのRFばく露(ROS産生実験では1時間ばく露、DNA損傷実験では3時間ばく露)を細胞に与えた。それぞれの実験は、(1)偽ばく露(対照)群、(2)RFばく露群、(3) FeCl2投与群、(4) FeCl2投与+RFばく露群の4群を比較した。その結果、FeCl2投与群では統計学的に有意な反応を示した;RFばく露群(CWまたはGSM類似信号のいずれも)では、ROS産生、DNA損傷、あるいは細胞生存率への影響が見られなかった、と報告している。

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研究目的(著者による)

To study the possible synergistic effects of radiofrequency exposure and ferrous chloride (iron ions were also used in previous studies: Brescia et al. 2009 and Zmyslony et al. 2004) on reactive oxygen species production and DNA damage.

詳細情報

Four groups were included: 1) sham exposure, 2) radiofrequency exposure, 3) chemical treatment, 4) chemical treatment and radiofrequency exposure. In the reactive oxygen species production experiments, the cells were exposed to radiofrequency and 10 µg/ml ferrous chloride for 1 h (group 4). In the comet assay experiments, the exposure time was 3 h and an additional chemical treatment (0.015% diethyl maleate (DEM)) was used to make DNA damage level observable. DEM decreases the cellular level of reduced glutathione (one of the main antioxidant systems against reactive oxygen species) and it was added 15 min before exposure to ferrous chloride and/or radiofrequency exposure.
Methyl methanesulfonate was used for positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 872 MHz
Modulation type: CW
ばく露時間: continuous for 1 h for the ROS production experiment; continuous for 3 hr for the comet assay experiment
ばく露2: 872 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h for the ROS production experiment; continuous for 3 hr for the comet assay experiment

General information

Cells were treated in four groups: i) sham exposure ii) RF radiation iii) chemical treatment iv) chemical treatment + RF irradiation

ばく露1

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h for the ROS production experiment; continuous for 3 hr for the comet assay experiment
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 248 mm x 175 mm x 420 mm aluminum waveguide with heat exchanger; cells placed on the glass surface of the heat exchanger
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

ばく露2

主たる特性
周波数 872 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h for the ROS production experiment; continuous for 3 hr for the comet assay experiment
Additional information GSM like field
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 5 W/kg - - - -

Reference articles

  • Höytö A et al. (2006): [適度の温度上昇はL929細胞のODC活性に影響する:低レベルのラジオ周波数では影響は見られない]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究の主なアウトカム(著者による)

The data indicate that neither intracellular reactive oxygen species production, DNA damage nor cell viability in human SH-SY5Y neuroblastoma cells were affected by continuous wave- or GSM-modulated radiofrequency exposure with or without co-exposure to ferrous chloride or ferrous chloride + DEM.

研究の種別:

研究助成

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