研究のタイプ: 医学/生物学の研究 (experimental study)

[Wistarラットリンパ球での915MHzマイクロ波放射誘導性DNA損傷に対するミツバチの毒液の防護効果:イン・ビトロ研究] med./bio.

Radioprotective effects of honeybee venom (Apis mellifera) against 915-MHz microwave radiation-induced DNA damage in wistar rat lymphocytes: in vitro study.

掲載誌: Int J Toxicol 2009; 28 (2): 88-98

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研究目的(著者による)

To study the radioprotective effect of bee venom against DNA damage induced by 915 MHz microwave irradiation in rat lymphocytes.

詳細情報

Honeybee venom is a complex mixture of different active components including peptides, enzymes and amines which have a variety of pharmaceutical properties. Bee venom is used in traditional medicine to treat a variety of diseases (e.g. arthritis, rheumatism, back pain) and it has been reported that it possess anti-mutagenic, pro-inflammatory, anti-inflammatory, anti-nociceptive and anti-cancer effects. Additionally it has been reported to possess a radioprotective capacity against gamma radiation.
Whole blood lymphocytes of Wistar rats are treated with 1 µg/ml bee venom four hours prior to and immediately before irradiation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 915 MHz
Modulation type: pulsed
ばく露時間: continuous for 30 min

ばく露1

主たる特性
周波数 915 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30 min
Modulation
Modulation type pulsed
Additional information

GSM basic signal modulation

ばく露装置
ばく露の発生源/構造
  • signal generator
ばく露装置の詳細 blood placed inside a certified gigahertz transversal electromagnetic mode cell
パラメータ
測定量 種別 Method Mass 備考
電界強度 30 V/m - - - uniform field inside the cell
電力密度 2.4 W/m² - - - -
SAR 0.6 W/kg - - whole body -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The standard comet assay revealed that microwave exposure increased the DNA damage in rat lymphocytes.
The Fpg-modified comet assay (as marker for oxidative stress induced DNA damage) revealed statistical differences compared to controls, suggesting that oxidative stress is a possible mechanism of DNA damage induction.
The data showed that bee venom has a radioprotective effect against basal and oxidative DNA damage. Bee venom alone (at a concentration of 1 µg/ml) did not have any impact on genotoxicity and did not produce oxidative damage in rat lymphocytes.

研究の種別:

研究助成

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