研究のタイプ: 医学/生物学の研究 (experimental study)

[GSM900 MHz携帯電話放射の連続ばく露と間欠ばく露による生物学的影響の比較:アポトーシス性細胞死の検出] med./bio.

Comparison of biological effects between continuous and intermittent exposure to GSM-900-MHz mobile phone radiation: Detection of apoptotic cell-death features.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2010; 700 (1-2): 51-61

この研究は、キイロショウジョウバエに毎日、6分間、GSM-900MHz携帯電話放射(EMR)を与え、6分間連続ばく露と合計6分間となる4種類の間欠ばく露(10分間間隔:1分ばく露×6回、2分ばく露×3回、3分ばく露×2回、3時間間隔:3分ばく露×2回)の影響とを比較し、また生殖能力およびアポトーシス細胞死誘導に対して、間欠ばく露が何らかの累積的影響を与えるか否かを調べた。著者は、以前の研究で「1日6分間の連続ばく露を5日間で、キイロショウジョウバエ生殖能力が大きく低下することを報告しており、今回はそれが間欠ばく露でも起こるかを検討した結果、10分間隔の間欠ばく露は、合計時間では同じになる連続ばく露とほぼ同等の影響を与えることが示されたが、ばく露間隔が3時間の場合は生殖器官に影響の回復時間を与えるような結果が見られたと報告している。

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研究目的(著者による)

To compare the influence of different patterns of intermittent exposures with different intervals, with the effects of continuous exposure of the same total duration of GSM exposure on the reproductive capacity of the fly Drosophila melanogaster.

詳細情報

The aim of the study was to examine whether the various intervals of intermittent exposures would indicate the possible existence of DNA repair mechanisms. A further aim of the study was to evaluate whether GSM irradiation has any effect on the disorganization of the actin-cytoskeleton network, which is another characteristic aspect of cell death in the egg chambers.
Each group consisted of nine female and nine male newly emerged flies kept in separate vials for the first 48 h. After the 48 h, when both males and females of each group were sexually mature, they were put together (nine pairs) in another glass vial and they were allowed to mate and lay eggs for the next 72 h. The flies were exposed by the different patterns of 6 min total daily exposure during the first 5 days of each experiment. On the sixth day there was an additional last exposure and 2-3 h later the parent fIies were removed from the vials. The flies were divided into five exposure groups (one continuous exposure group and four intermittent exposure groups) and one sham exposure group.
For cell death investigation ovarioles were investigated (background information about ovarioles, see Panagopoulos 2007b).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 6 min./day on 6 days
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: 1 min. on - 10 min. off, repeated 6 times/day on 6 days
ばく露3: 900 MHz
Modulation type: pulsed
ばく露時間: 2 min. on - 10 min. off, repeated 3 times/day on 6 days
ばく露4: 900 MHz
Modulation type: pulsed
ばく露時間: 3 min. on - 10 min. off, repeated 2 times/day on 6 days
ばく露5: 900 MHz
Modulation type: pulsed
ばく露時間: 3 min. on - 6 hrs off, repeated 2 times/day on 6 days
ばく露6:
  • unspecified
ばく露時間: 6 min./day on 6 days
sham exposure

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 6 min./day on 6 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
ばく露装置の詳細 mobile phone's antenna in contact with the glass vial containing the insects; phone in speaking mode
パラメータ
測定量 種別 Method Mass 備考
電界強度 18.6 V/m average over time 測定値 - +/- 2.7 V/m
磁束密度 0.075 µT average over time 測定値 - +/- 0.017 µT
電力密度 0.35 mW/cm² average over time 測定値 - +/-0.07 mW/cm²

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 1 min. on - 10 min. off, repeated 6 times/day on 6 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 18.6 V/m average over time 測定値 - +/- 2.7 V/m
磁束密度 0.075 µT average over time 測定値 - +/- 0.017 µT
電力密度 0.35 mW/cm² average over time 測定値 - +/-0.07 mW/cm²

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 2 min. on - 10 min. off, repeated 3 times/day on 6 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 18.6 V/m average over time 測定値 - +/- 2.7 V/m
磁束密度 0.075 µT average over time 測定値 - +/- 0.017 µT
電力密度 0.35 mW/cm² average over time 測定値 - +/-0.07 mW/cm²

ばく露4

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 3 min. on - 10 min. off, repeated 2 times/day on 6 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 18.6 V/m average over time 測定値 - +/- 2.7 V/m
磁束密度 0.075 µT average over time 測定値 - +/- 0.017 µT
電力密度 0.35 mW/cm² average over time 測定値 - +/-0.07 mW/cm²

ばく露5

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 3 min. on - 6 hrs off, repeated 2 times/day on 6 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 18.6 V/m average over time 測定値 - +/- 2.7 V/m
磁束密度 0.075 µT average over time 測定値 - +/- 0.017 µT
電力密度 0.35 mW/cm² average over time 測定値 - +/-0.07 mW/cm²

ばく露6

主たる特性
周波数
  • unspecified
ばく露時間 6 min./day on 6 days
Additional information sham exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 0 V/m - - - -
磁束密度 0 µT - - - -
電力密度 0 mW/cm² - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that intermittent exposure decreased the reproductive capacity and altered the actin-cytoskeleton network of the egg chambers, and that the effect is also due to DNA fragmentation. Intermittent exposures with 10 minutes intervals between exposure sessions were almost equally effective as continuous exposure of the same total duration, whereas longer intervals between the exposures seemed to allow the flies to recover and partly overcome the above-mentioned effects of the GSM exposure.

研究の種別:

研究助成

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