研究のタイプ: 医学/生物学の研究 (experimental study)

[磁界処理されたヒト神経膠腫細胞における遺伝子およびタンパク質発現の変化] med./bio.

Changes in gene and protein expression in magnetic field-treated human glioma cells.

掲載誌: Toxicol Mech Methods 2005; 15 (2): 115-120

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研究目的(著者による)

The possible carcinogenicity of magnetic fields should be investigated by examining the changes in gene expression and protein expression and their relationship in human glioma cells after an exposure to a 60 Hz magnetic field.

詳細情報

Triplicate sets of cell cultures were exposed to the magnetic field or to sham exposure, respectively. Biomarkers of carcinogens should be detected with protein expression analysis, while the parallel gene expression analysis should identify related oncogenes.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 3 h

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 3 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two concentric Merrit/Helmholtz coil systems, inner coils for exposure, outer coils to limit stray fields; modified commercial incubator placed as exposure chamber in the coil system
Sham exposure A sham exposure was conducted.
Additional information for sham exposure: double-wound coils were energized with opposing currents so that the resulting magnetic field was zero
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.2 µT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The results of gene expression analysis showed a significant up-regulation of five and down-regulation of 25 genes in cells exposed to the magnetic field compared to sham exposure. Protein expression analysis indicated that the expression rates of ten identified proteins were significantly altered. Three of these showed an increase and seven a decrease in their expression rate, following the exposure compared to sham exposure. However, no obvious relationship between the affected genes and proteins could be found.
The authors conclude that there is an effect of 60 Hz magnetic fields on gene expression and protein expression of human glioma cells, but that further studies are needed to clarify and evaluate the relationships.

研究の種別:

研究助成

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