この研究は、ヒト神経膠腫SF767細胞に60 Hz磁界（擬似または1.2 μT）への3時ばく露を与え、その影響を評価するためにタンパク質プロファイルと遺伝学的変化を調べた。マイクロアレイ法を用いて遺伝子の変化を決定し、その変化が細胞タンパク質変化の前兆であるか否かを評価した。その結果、磁界ばく露群では、5個の遺伝子の発現が増加したのに対し、25個の遺伝子発現が減少を示した；同定された10個のタンパク質の平均存在量は、ばく露群において、擬似ばく露群に比べて変化した（増加が3、減少が7）、と報告している。
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The possible carcinogenicity of magnetic fields should be investigated by examining the changes in gene expression and protein expression and their relationship in human glioma cells after an exposure to a 60 Hz magnetic field.
Triplicate sets of cell cultures were exposed to the magnetic field or to sham exposure, respectively. Biomarkers of carcinogens should be detected with protein expression analysis, while the parallel gene expression analysis should identify related oncogenes.
ばく露時間: continuous for 3 h
|ばく露時間||continuous for 3 h|
|ばく露装置の詳細||two concentric Merrit/Helmholtz coil systems, inner coils for exposure, outer coils to limit stray fields; modified commercial incubator placed as exposure chamber in the coil system|
|Sham exposure||A sham exposure was conducted.|
|Additional information||for sham exposure: double-wound coils were energized with opposing currents so that the resulting magnetic field was zero|
The results of gene expression analysis showed a significant up-regulation of five and down-regulation of 25 genes in cells exposed to the magnetic field compared to sham exposure. Protein expression analysis indicated that the expression rates of ten identified proteins were significantly altered. Three of these showed an increase and seven a decrease in their expression rate, following the exposure compared to sham exposure. However, no obvious relationship between the affected genes and proteins could be found.
The authors conclude that there is an effect of 60 Hz magnetic fields on gene expression and protein expression of human glioma cells, but that further studies are needed to clarify and evaluate the relationships.