研究のタイプ: 医学/生物学の研究 (experimental study)

[高周波電磁界(GSM 1.8 GHz)から生じるヒト栄養膜細胞株HTR-8/SVNeoにおける過渡的DNA損傷のアルカリ・コメットアッセイでの評価] med./bio.

Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay.

掲載誌: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2010; 683 (1-2): 35-42

著者らは以前の論文で、携帯電話で大規模に使われている振幅変調1.8 GHz正弦波GSM-217 HzSAR=2 W/kg)はヒトの栄養膜細胞株HTR-8/SVneoに原発性DNA損傷のレベル上昇を生じないことを報告している。今回は、信号のタイプ・ばく露時間を変えて、同じ細胞株で実験した。用いた信号は、1.8 GHzの連続波(CW)、振幅変調のGSM-217 Hz信号およびGSM-Talk信号の断続的ばく露(5分間:オン、10分間:オフ)で、それぞればく露時間は4、16、24時間とした。その結果、非変調のCWは無効果であったが、振幅変調した2種類の信号への16および24時間ばく露はコメット・パラメーターの有意な増加を誘導した。ただし、その変化は2時間以内に擬似ばく露と同じレベルにまで修復されたと報告している。

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研究目的(著者による)

To determine whether radiofrequency electromagnetic fields could induce genotoxic effects in trophoblast cells. Possible effects of radiofrequency electromagnetic fields on DNA integrity were evaluated in the same cell model as in a previous study (Valbonesi et al. 2008) after intermittent exposure to 1.8 GHz continuous wave and to two different GSM signals (GSM-217 and GSM-Talk) for 4, 16, and 24 h.

詳細情報

To study whether radiofrequency electromagnetic fields have no significant effects or whether cells are able to compensate the potential consequences, the authors also investigated the ability of the cells to repair the potential DNA alterations within a post-irradiation recovery period of 30 and 120 min (DNA repair).
Hydrogen peroxide treatment was used for the positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: CW
ばく露時間: 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr
ばく露2: 1.8 GHz
Modulation type: pulsed
ばく露時間: 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr
ばく露3: 1.8 GHz
Modulation type: pulsed
ばく露時間: 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 128.5 mm x 65 mm x 424 mm brass single-mode waveguide resonator inside an incubator; plastic holder for hosting the petri dishes in two stacks placed in the rsonator; dishes positioned in the H-field maximum of the standing wave inside the waveguide
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - -

ばく露2

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr
Additional information GSM-217
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

modulation frequency: 8 Hz

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - -

ばく露3

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 5 min on / 10 min off - for 4 hr, 16 hr or 24 hr
Additional information GSM-Talk
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

combination of GSM-217 und GSM-DTX modulation frequencies: 2 Hz, 8 Hz and 217 Hz

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The amplitude modulated signals GSM-217 and GSM-Talk induced a significant increase in the comet assay parameters in trophoblast cells, while the un-modulated continuous wave exposure was ineffective. However, alterations were rapidly recovered and the DNA integrity of exposed cells was similar to that of sham exposed cells within two hours of recovery in the absence of irradiation.
The data suggest that radiofrequency electromagnetic field exposure with a carrier frequency and modulation scheme typical of the GSM signal may affect the DNA integrity.

研究の種別:

研究助成

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