研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界ばく露による水迷路タスク成績の欠陥および海馬と線条体での酸化ストレス] med./bio.

Deficits in water maze performance and oxidative stress in the hippocampus and striatum induced by extremely low frequency magnetic field exposure.

掲載誌: PLoS One 2012; 7 (5): e32196

この実験研究はマウスを用いて、2つの水迷路タスクによる学習海馬および線条体酸化ストレスを表すパラメータに対する超低周波磁界の影響を調べた。72匹の成獣C57BL/6マウスを24匹ずつの3群(I群:擬似ばく露、II群:50 Hz, 1 mT ばく露、III群:50 Hz, 0.1 mT ばく露)に分け、4時間/日、連続12週間のばく露擬似ばく露)を行った。ばく露セッション終了後、水迷路を使った学習タスク(空間学習タスク:各群12匹、学習習慣化タスク:各群12匹)を4試行/日で4日間実施し、その後、脳を摘出し、海馬及び線条体組織を均質化して酸化ストレスパラメータを測定した。その結果、1 mT ばく露群において、海馬及び線条体に強い酸化ストレス誘導されていた;海馬が関連する空間学習および線条体が関連する学習習慣化に欠陥があったとの所見を報告している

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研究目的(著者による)

To study the effects of chronic extremely low frequency magnetic field exposure of mice on habit learning which is dependent on striatum, and on spatial learning which is dependent on hippocampus, as well as to evaluate some parameters indicative for oxidative stress in these two structures.

詳細情報

72 male mice were divided into three groups (each group n=24): one sham exposure group and two exposure groups (1 mT and 0.1 mT).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4 h/day for 12 weeks
ばく露2: 50 Hz
ばく露時間: continuous for 4 h/day for 12 weeks

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 h/day for 12 weeks
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 coil of four layers of 250 turns of 1.68 mm copper wire wrapped horizontally around a 70 cm x 40 cm x 43 cm plastic frame; exposure area inside the coil (60 cm x 30 cm x 43 cm); mice were housed in pairs in plexiglas cages (50 cm x 25 cm x 25 cm) on a freestanding wood
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 h/day for 12 weeks
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -

Reference articles

  • Sun H et al. (2010): [1日齢のヒヨコの一試行受動的回避学習に対する50Hz磁界への出生前のばく露の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

There was no significant difference in body weight gain and locomotor activity across the three groups. However, animals exposed to the extremely low frequency magnetic field (1 mT) took longer to find the platform in the water maze compared with the other two groups. No significant effect was found in animals exposed at 0.1 mT.
Extremely low frequency magnetic field exposure (0.1 mT) did not result in the development of oxidative stress in mice hippocampus or striatum. However, exposure at 1 mT induced oxidative stress in mice hippocampus and striatum: The levels of malondialdehyde were significantly increased compared with the other two groups. The values of catalase and glutathione peroxidase enzyme activities as well as the total antioxidant capacity were significantly decreased compared with the other two groups.
In conclusion, extremely low frequency magnetic field exposure (1 mT) induced oxidative stress in the hippocampus and striatum and impaired hippocampal-dependent spatial learning and striatum-dependent habit learning. The data provide evidence for the association between the impairment of learning and oxidative stress in hippocampus and striatum induced by extremely low frequency magnetic field exposure.

研究の種別:

研究助成

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