この実験研究はマウスを用いて、2つの水迷路タスクによる学習、海馬および線条体の酸化ストレスを表すパラメータに対する超低周波磁界の影響を調べた。72匹の成獣C57BL/6マウスを24匹ずつの3群（I群：擬似ばく露、II群：50 Hz, 1 mT ばく露、III群：50 Hz, 0.1 mT ばく露）に分け、4時間／日、連続12週間のばく露（擬似ばく露）を行った。ばく露セッション終了後、水迷路を使った学習タスク（空間学習タスク：各群12匹、学習の習慣化タスク：各群12匹）を4試行／日で4日間実施し、その後、脳を摘出し、海馬及び線条体の組織を均質化して酸化ストレスパラメータを測定した。その結果、1 mT ばく露群において、海馬及び線条体に強い酸化ストレスが誘導されていた；海馬が関連する空間学習および線条体が関連する学習の習慣化に欠陥があったとの所見を報告している
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To study the effects of chronic extremely low frequency magnetic field exposure of mice on habit learning which is dependent on striatum, and on spatial learning which is dependent on hippocampus, as well as to evaluate some parameters indicative for oxidative stress in these two structures.
72 male mice were divided into three groups (each group n=24): one sham exposure group and two exposure groups (1 mT and 0.1 mT).
|ばく露時間||continuous for 4 h/day for 12 weeks|
|ばく露装置の詳細||coil of four layers of 250 turns of 1.68 mm copper wire wrapped horizontally around a 70 cm x 40 cm x 43 cm plastic frame; exposure area inside the coil (60 cm x 30 cm x 43 cm); mice were housed in pairs in plexiglas cages (50 cm x 25 cm x 25 cm) on a freestanding wood|
|Sham exposure||A sham exposure was conducted.|
There was no significant difference in body weight gain and locomotor activity across the three groups. However, animals exposed to the extremely low frequency magnetic field (1 mT) took longer to find the platform in the water maze compared with the other two groups. No significant effect was found in animals exposed at 0.1 mT.
Extremely low frequency magnetic field exposure (0.1 mT) did not result in the development of oxidative stress in mice hippocampus or striatum. However, exposure at 1 mT induced oxidative stress in mice hippocampus and striatum: The levels of malondialdehyde were significantly increased compared with the other two groups. The values of catalase and glutathione peroxidase enzyme activities as well as the total antioxidant capacity were significantly decreased compared with the other two groups.
In conclusion, extremely low frequency magnetic field exposure (1 mT) induced oxidative stress in the hippocampus and striatum and impaired hippocampal-dependent spatial learning and striatum-dependent habit learning. The data provide evidence for the association between the impairment of learning and oxidative stress in hippocampus and striatum induced by extremely low frequency magnetic field exposure.