研究のタイプ: 医学/生物学の研究 (experimental study)

[低周波高磁界にばく露されたマウスの脾臓のL-カルニチンとq10における免疫調節効果] med./bio.

Immunomodulatory effects of L-carnitine and q10 in mouse spleen exposed to low-frequency high-intensity magnetic field.

掲載誌: Toxicology 2003; 187 (2-3): 171-181

この研究は、マウスのいくつかの免疫パラメーターに対する低周波(50 Hz)高強度(20 mT = 200 G)電磁界EMF)への反復ばく露の影響を調べた。動物は、1日1回30分間のばく露を、週に3回、2週間受けた。また、非特異的免疫刺激効果を持つ2つの抗ラジカル物質、L-カルニチン(200 mg / kg;腹腔内投与)およびQ10(200 mg / kg;経口投与)の免疫調節効果の可能性についても調べた。二つの薬剤はそれぞれ、各EMFばく露の1時間前に投与された。免疫のエンドポイントは、総体重、脾臓/体重比、脾細胞生存率、全白血球数および分画(リンパ球単球好中球)、マイトジェン(フィトヘマグルチニン(PHA)、コンカナバリンA(Con-A)、リポプロサッカライド(LPS))によって誘発されるリンパ球増殖である。その結果、磁界ばく露は、脾細胞の生存率、全白血球数、マイトジェン誘発リンパ球増殖を減少させた;検査した免疫パラメーターの大部分へのEMFの悪影響を、L-カルニチンは改善する可能性が見られたが、Q-10では見られなかった、と報告している。

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研究目的(著者による)

The effects of exposure of mice to a 50 Hz magnetic field and of a co-exposure with two immunostimulating substances on some immune parameters should be investigated.

詳細情報

Mice were divided into the following groups (n=6 each): 1) sodium chloride solution administration and exposure to the magnetic field, 2) coenzyme Q10 administration (200 mg/kg oral) and exposure and 3) L-carnitine administration (200 mg/kg intraperitoneal) and exposure. For each exposure group, a respective control group with the same administrations but without exposure was used. Administrations were conducted every second day 1 hour before the start of exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 30 min/day, 3 days/week (every other day) for 2 weeks

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 30 min/day, 3 days/week (every other day) for 2 weeks
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 three rectangular coils (each 22 cm x 12 cm with 760 turns of 1 mm copper wire) connected in parallel and wrapped around a wooden frame; up to 12 mice were placed inside the coil system, which was closed at both ends with plexiglass windows
パラメータ
測定量 種別 Method Mass 備考
磁束密度 20 mT - 測定値 - vertical field

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

Injections with salt solutions and exposure to the magnetic field (group 1) resulted in a significantly decreased spleen cell viability, proliferation of T lymphocytes and B lymphocytes, total count of white blood cells and body weight and a significantly increased amount of monocytes compared to the respective control group.
When injected with L-carnitine and exposed to the magnetic field (group 3), almost all these effects vanished and no significant differences were observed compared to the salt injected control group anymore. This protective effect was not detected in mice injected with coenzyme Q10 and exposure to the magnetic field (group 2).
The authors conclude that exposure of mice to a 50 Hz magnetic field might have adverse effects on the immune system and that co-exposure with L-carnitine, but not with coenzyme Q10, could mitigate these effects.

研究の種別:

研究助成

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